Technology Platforms for 3D Cell Culture (eBook)

A User's Guide

Stefan Przyborski (Herausgeber)

eBook Download: EPUB

2017
John Wiley & Sons (Verlag)
978-1-118-85153-1 (ISBN)

Infos

Technology Platforms for 3D Cell Culture: A Users Guide points to the options available to perform 3D culture, shows where such technology is available, explains how it works, and reveals how it can be used by scientists working in their own labs.

• Offers a comprehensive, focused guide to the current state-of-the-art technologies available for 3D cell culture
• Features contributions from leading developers and researchers active in 3D cell technology
• Gives clear instruction and guidance on performing specific 3D culture methods, along with colour illustrations and examples of where such technologies have been successfully applied
• Includes information on resources and technical support to help initiate the use of 3D culture methods

Stefan Przyborski is Professor of Cell Technology in the Department of Biosciences, Durham University, UK. He is also the Chief Scientific Officer for ReproCELL Europe, UK. A recognised leader in the field of 3D cell culture, Professor Przyborski has published extensively in this field and presents his work regularly at international events.

Inhalt

List of contributors, vii

Preface, xi

List of abbreviations, xiv

1 An introduction to the third dimension for routine cell culture, 1
Antonio Romo-Morales and Stefan Przyborski

Part I: Aggregate-based technologies

2 Gravity-enforced microtissue engineering, 23
Randy Strube, Johannes Haugstetter, Markus Furter, Andreia Fernandez, David Fluri and Jens M. Kelm

3 Physiologically relevant spheroid models for three-dimensionalcell culture, 50
Nicole A. Slawny and MaryAnn Labant

4 NanoCulture Plate: A scaffold-based high-throughput three-dimensional cell culture system suitable for live imaging and co-culture, 74
Manabu Itoh, Kazuya Arai, Hiromi Miura and M. Mamunur Rahman

5 Micro-moulded non-adhesive hydrogels to form multicellular microtissues - the 3D Petri Dish¯®, 97
Elizabeth Leary, Sean Curran, Michael Susienka, Kali L. Manning, Andrew M. Blakely and Jeffrey R. Morgan

6 Organotypic microtissues on an air-liquid interface, 123
Lars E. Sundstrom, Igor Charvet and Luc Stoppini

Part II: Hydrogels

7 Materials and assay systems used for three-dimensional cell culture, 145
Suparna Sanyal and Marshall Kosovsky

8 HyStem¯®, a customisable hyaluronan-based hydrogel matrix for 3D cell culture, 173
T. I. Zarembinski, B. J. Engel, N. J. Doty, P. E. Constantinou, M. V. Onorato, I. E. Erickson, E. L. S. Fong, M. Martinez, R. L. Milton, B. P. Danysh, N. A. Delk, D. A. Harrington, M. C. Farach-Carson and D. D. Carson

9 3-D Life biomimetic hydrogels: A modular system for cell environment design, 197
Brigitte M. Angres and Helmut Wurst

Part III: Scaffolds

10 Alvetex¯®, a highly porous polystyrene scaffold for routine three-dimensional cell culture, 225
Antonio Romo-Morales, Eleanor Knight and Stefan Przyborski

11 CelluSponge(TM) and Go Matrix as innovative three-dimensional cell culture platforms, 250
Bramasta Nugraha

12 Mimetix¯® electrospun scaffold: An easy-to-use tool for 3D cell culture in drug discovery and regenerative medicine, 284
Robert J. McKean and Elena Heister

Part IV: 3D bioreactor technologies

13 Quasi Vivo¯® bioreactor technology, 305
J. Malcolm Wilkinson

14 Three-dimensional cell-based assays in hollow fibre bioreactors, 327
John J. S. Cadwell and William G. Whitford

15 Three-dimensional engineered tissues for high-throughput compound screening: Mechanical properties of skin and ageing, 351
Michael Conway, Ayla Annac and Tetsuro Wakatsuki

16 Three-dimensional cell culture in the Rotary Cell Culture System(TM), 370
Stephen S. Navran

Glossary, 386

Index, 393

Textauszug

List of abbreviations

2D	Two‐dimensional

3D	Three‐dimensional

5‐FU	5‐Fluorouracil

A1AT	α1 Antitrypsin

ADME Tox

Absorption, distribution, metabolism, excretion and toxicity

ALI	Air‐liquid interface

ASC	Adipose‐derived stem cells

ATP	Adenosine‐5’‐triphosphate

ATRA	All trans‐retinoic acid

BBB	Blood–brain barrier

bFGF	Basic fibroblast growth factor

BME	Basement membrane extracts

BNDF	Brain‐derived neurotrophic factor

CAF	Cancer‐associated fibroblast

CD	Cytochalasin D; cluster of differentiation

CDI	1,1’‐Carbonyldiimidazole

CDM‐HD

Chemically defined medium for high‐density cell culture

C/EBPa

CCAAT‐enhancer‐binding proteins

CHO	Chinese hamster ovary

CLL	Chronic lymphocytic leukaemia

CNS	Central nervous system

CP	Cortical plate

CTG	CellTracker green; CellTiter‐Glo

CV	Coefficient of variation

CYP1A1

Cytochrome P450, family 1, subfamily A, polypeptide 1

CYP2E1

Cytochrome P450 2E1

CYP3A4

Cytochrome P450 3A4

d4T	2′,3′‐Didehydro‐3′deoxythymidine

DNA	Deoxyribonucleic acid

DCIS	Ductal carcinoma in situ

DLS	Dynamic light scattering

DMEM	Dulbecco’s modified eagle medium

DMSO	Dimethyl sulfoxide

DNP	Dinitrophenol

DOX	Doxorubicin

DPMK	Drug metabolism and pharmacokinetics

DRG	Dorsal root ganglia

DTT	Dithiothreitol

EthD	Ethidium homodimer‐1

EB	Embryoid body

ECM	Extracellular matrix

ECS	Extracapillary space

EDTA	Ethylenediamine tetra‐acetic acid

EEF1	Elongation factor 1 complex

EFP	Evoked field potential

EGF	Epidermal growth factor

EGFR	Epidermal growth factor receptor

EHS	Engelbreth‐Holm‐Swarm

EHT	Engineered heart tissue

ELISA

Enzyme‐linked immunosorbent assay

EMT	Epithelial‐mesenchymal transition

ESC	Embryonic stem cell

ET	Engineered tissue

EVS	Extravascular space

FA	Focal adhesion

FACS	Fluorescence activated cell sorting

FBS	Fetal bovine serum

FDA	Food and Drug Administration; fluorescein diacetate

GABA	γ‐Aminobutyric acid

GAG	Glycosaminoglycan

GFAP	Glial fibrillary acid protein

GFP	Green fluorescent protein

GMP	Good Manufacturing Practice

GSH	Glutathione synthase

GSSG	Oxidised glutathione

HA	Hyaluronic acid

HARV	High aspect ratio vessel

HCA	High‐content analysis

HCV	Hepatitis C virus

HCVpp

Hepatitis C virus pseudoparticles

HD	Hanging drop

HDMEC

Human dermal microvascular endothelial cell

HDP	Hanging drop plate

H&E	Haematoxylin and eosin

HF	Hollow fibre

HFBR	Hollow fibre bioreactor

HFIM	Hollow fibre infection model

HIPE	High internal phase emulsion

HiPSC

Human induced pluripotent stem cell

HIV	Human immunodeficiency virus

HMEC	Human microvascular endothelial cell

hMSC	Human mesenchymal stem cell

HNF4α

Hepatocyte nuclear factor 4 α

HPC	Hydroxypropylcellulose

HSC	Haematopoietic stem cell

HS	Heparan sulfate

HTS	High‐throughput screening

HUVEC

Human umbilical vein endothelial cell

ICM	Inner cell mass

ID	Internal diameter

IDC	Invasive ductal carcinoma

IgG	Immunoglobulin G

iPSC	Induced pluripotent stem cell

LC‐MS

Liquid chromatography‐mass spectrometry

LCST	Liquid crystal solution temperature

LDH	Lactate dehydrogenase

NF	Neurofilament

M‐dPEG‐NHS

Maleimide‐dPEG8‐N‐hydroxysuccinimide ester

Mal‐PVA

Maleimide‐functionalised polyvinyl alcohol

MAP	Microtubule‐associated protein

MCR	Multicellular...

Produkt-Details

• Erscheint lt. Verlag: 3.3.2017
• Sprache: englisch
• Themenwelt: Medizin / Pharmazie ► Gesundheitsfachberufe
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete
• Themenwelt: Naturwissenschaften ► Biologie ► Zellbiologie
• Themenwelt: Technik ► Umwelttechnik / Biotechnologie
• Schlagworte: 2D cell culture • 3d cell culture • biomedical engineering • Biomedizinische Technik • Biomedizintechnik • Biotechnologie • Biotechnologie i. d. Biowissenschaften • Biotechnology • Biowissenschaften • cancer cell biology • cell biologists • Cell Biology • Life Sciences • Medical Cell Biology • Medizinische Zellbiologie • Stem Cells • Tissue, Cell, and Genetic Engineering • Tissue engineering • Zell-, Gewebe- u. Gen-Engineering • Zellkultur
• ISBN-10: 1-118-85153-6 / 1118851536
• ISBN-13: 978-1-118-85153-1 / 9781118851531