How Free Cationic Polymer Chains Promote Gene Transfection

Yue Yanan (Autor)

Buch | Softcover

XVIII, 94 Seiten

2016 | Softcover reprint of the original 1st ed. 2013
Springer International Publishing (Verlag)
978-3-319-34378-5 (ISBN)

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In this PhD thesis, Yue Yanan addresses a long-overlooked and critical question in the development of non-viral vectors for gene delivery. The author determines that those uncomplexed and cationic polymer chains free in the solution mixture of polymer and DNA facilitate and promote gene transfection. Furthermore, by using a combination of synthetic chemistry, polymer physics and molecular biology, Yue confirms that it is those cationic polymer chains free in the solution mixture, rather than those bound to DNA chains, that play a decisive role in intracellular trafficking. Instead of the previously proposed and widely accepted "proton sponge" model, the author's group propose a new hypothesis based on the results of several well-designed and decisive experiments. These results show that free polycationic chains with a length of more than ~10 nm are able to partially block the fusion between different endocytic vesicles, including the endocytic-vesicle-to-endolysosome pathway. This thesis is highly original and its results greatly deepen our understanding of polymer-mediated gene transfection. More importantly, it provides new insights into the rational design of next-generation superior polymeric gene-delivery vectors.

Introduction and background.- Revisiting complexation between DNA and polyethylenimine: the effect of uncomplexed chains free in the solution mixture on gene transfection.- Revisiting complexation between DNA and polyethylenimine: the effect of length of free polycationic chains on gene transfection.- Quantitative comparison of endocytosis and intracellular trafficking of DNA/polymer complexes in the absence/presence of free polycationic chains.

Erscheinungsdatum	29.08.2016
Reihe/Serie	Springer Theses
Zusatzinfo	XVIII, 94 p. 45 illus., 38 illus. in color.
Verlagsort	Cham
Sprache	englisch
Maße	155 x 235 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Chemie ► Organische Chemie
	Technik ► Maschinenbau
Schlagworte	biochemistry • Chemistry and Materials Science • Cyclodextrin-Based Polymers • Endocytic Vescicles • Endocytic-Vesicle-to-Endolysosome Pathway • Free Polycationic Chains • Gene-Delivery Vectors • Gene Transfection • Intracellular trafficking • Nucleic Acid Chemistry • Organic Chemistry • polymer chemistry • Polymer-Mediated Gene Transfection • Polymer sciences • Polyplex • Proton Sponge
ISBN-10	3-319-34378-5 / 3319343785
ISBN-13	978-3-319-34378-5 / 9783319343785
Zustand	Neuware