Advances in the Understanding of Biological Sciences Using Next Generation Sequencing (NGS) Approaches (eBook)

Gaurav Sablok, Sunil Kumar, Saneyoshi Ueno, Jimmy Kuo, Claudio Varotto (Herausgeber)

eBook Download: PDF

2015 | 1. Auflage
XII, 241 Seiten
Springer-Verlag
978-3-319-17157-9 (ISBN)

Infos

Provides a global view of the recent advances in the biological sciences and the adaption of the pathogen to the host plants revealed using NGS. Molecular Omic’s is now a major driving force to learn the adaption genetics and a great challenge to the scientific community, which can be resolved through the application of the NGS technologies. The availability of complete genome sequences, the respective model species for dicot and monocot plant groups, presents a global opportunity to delineate the identification, function and the expression of the genes, to develop new tools for the identification of the new genes and pathway identification. Genome-wide research tools, resources and approaches such as data mining for structural similarities, gene expression profiling at the DNA and RNA level with rapid increase in available genome sequencing efforts, expressed sequence tags (ESTs), RNA-seq, gene expression profiling, induced deletion mutants and insertional mutants, and gene expression knock-down (gene silencing) studies with RNAi and microRNAs have become integral parts of plant molecular omic’s. Molecular diversity and mutational approaches present the first line of approach to unravel the genetic and molecular basis for several traits, QTL related to disease resistance, which includes host approaches to combat the pathogens and to understand the adaptation of the pathogen to the plant host. Using NGS technologies, understanding of adaptation genetics towards stress tolerance has been correlated to the epigenetics. Naturally occurring allelic variations, genome shuffling and variations induced by chemical or radiation mutagenesis are also being used in functional genomics to elucidate the pathway for the pathogen and stress tolerance and is widely illustrated in demonstrating the identification of the genes responsible for tolerance in plants, bacterial and fungal species.

Autor

Guarav Sablok, PhDResearch Fellow, Plant Functional Biology and Climate Change Cluster (C3)University of Technology, SydneyPO Box 123 Broadway NSW 2007 AustraliaT:: 0039-3270484-732Email: [e-mail] Kumar, M.Sc ,IPR, MCA, Ph.DSenior Scientist, Department of Bioinformatics Institute of Life Sciences Nalco Square, C S PurBhubaneswar, Odisha 275103, India T: 91-6742301500F: 91-6742300728E-mail: [e-mail] Jimmy Kuo, Ph.DAssociate Researcher, National Museum of Marine Biology and AquariumGraduate Institute of Marine Biotechnology, National Dong Haw University2 Houwan Road, ChechengPingtung 944, TaiwanTel: 886-8-8825001 ext. 1358Fax: [phone]E-mail: [e-mail] Ueno, Ph.DDepartment of Forest Genetics 1 Matsunosato, TsukubaIbaraki 3058687, JapanT: [phone]F: [phone]Email: [e-mail] Varotto, Msc, Ph.DDepartment of Biodiversity and Molecular Ecology, Research and Innovation CenterFondazione Edmund Mach, Research and Innovation CenterVia E. Mach 1, 38010 San Michele all’Adige (TN), ItalyT: [phone]F: [phone]Email: [e-mail]

Inhalt

Ch. 1: Expression analysis and genome annotations with RNA sequencing             Masaaki Kobayashi, Hajime Ohyanagi, Kentaro Yano Ch. 2: The application of Next Generation Sequencing techniques to Plant Epigenomics             Manu J. Dubin Ch. 3: Whole genome sequencing to identify genes and QTL in rice Ryohei Terauchi, Akira Abe, Hiroki Takagi, Muluneh Tamiru, Rym Fekih, Satoshi Natsume, Hiroki Yaegashi, Shunichi Kosugi, Hiroyuki Kanzaki, Hideo Matsumura, Hiromasa Saitoh, Kentaro Yoshida, Liliana Cano, Sophien Kamoun Ch. 4: Variant calling using NGS data in European aspen (Populus tremula) Jing Wang, Douglas Scofield, Nathaniel R. Street, Pär K. Ingvarsson Ch. 5: Leafy Spurge Genomics: A Model Perennial Weed To Investigate Development, Stress Responses, And Invasiveness David Horvath, James Anderson, Wun Chao, Michael Foley, Münevver Doğramaci   Ch. 6: Utilization of NGS and proteomic-based approaches to gain insights on cellular responses to singlet oxygen and improve energy yields for bacterial stress adaptation Roger Greenwell, Mobashar Hussain Urf Turabe Fazil, H P Pandey. Ch. 7: Experimental evolution and next generation sequencing illuminate the evolutionary trajectories of microbes Mario Fares    Ch. 8: Plant carbohydrate active enzyme (CAZyme) repertoires: a comparative Study Huansheng Cao, Alex Ekstrom, Yanbin Yin Ch. 9: Metagenomics of Plant- Microbe Interactions Riccardo Rosselli, Andrea Squartini             Ch. 10: Genes and trans-factors underlying embryogenic transition in plant soma-cells Dhananjay K. Pandey, Bhupendra Chaudhary              Ch. 11: Bioinformatics tools to analyze the proteome and genome data                         Ritesh Kumar, Shalini Singh, Vikash Kumar Dubey              Ch. 12: High through-put transcriptome analysis of plant stress responses                         Hüseyin Tombuloğlu, Güzin Tombuloğlu                         Ch. 13: CNV and structural variation in plants: prospects of NGS                       Approaches                       Enrico Francia, Nicola Pecchioni, Alberto Policriti, Simone Scalabrin

Produkt-Details

• Erscheint lt. Verlag: 16.7.2015
• Zusatzinfo: XII, 241 p. 40 illus., 31 illus. in color.
• Verlagsort: Cham
• Sprache: englisch
• Themenwelt: Naturwissenschaften ► Biologie ► Botanik
• Themenwelt: Technik
• Schlagworte: deletion mutants • expressed sequence tags • genome shuffling • insertional mutants • Pathway identification
• ISBN-10: 3-319-17157-7 / 3319171577
• ISBN-13: 978-3-319-17157-9 / 9783319171579