Instrumental Analysis of Intrinsically Disordered Proteins (eBook)

Assessing Structure and Conformation

Vladimir Uversky, Sonia Longhi (Autoren)

eBook Download: PDF

2011 | 1. Auflage
736 Seiten
John Wiley & Sons (Verlag)
978-0-470-60260-7 (ISBN)

Infos

Instrumental techniques for analyzing intrinsically disordered
proteins

The recently recognized phenomenon of protein intrinsic disorder
is gaining significant interest among researchers, especially as
the number of proteins and protein domains that have been shown to
be intrinsically disordered rapidly grows. The first reference to
tackle this little-documented area, Instrumental Analysis of
Intrinsically Disordered Proteins: Assessing Structure and
Conformation provides researchers with a much-needed,
comprehensive summary of recent achievements in the methods for
structural characterization of intrinsically disordered proteins
(IDPs).

Chapters discuss:

* Assessment of IDPs in the living cell

* Spectroscopic techniques for the analysis of IDPs, including NMR
and EPR spectroscopies, FTIR, circular dichroism, fluorescence
spectroscopy, vibrational methods, and single-molecule analysis

* Single-molecule techniques applied to the study of IDPs

* Assessment of IDP size and shape

* Tools for the analysis of IDP conformational stability

* Mass spectrometry

* Approaches for expression and purification of IDPs

With contributions from an international selection of leading
researchers, Instrumental Analysis of Intrinsically Disordered
Proteins: Assessing Structure and Conformation fills an
important need in a rapidly growing field. It is required reading
for biochemists, biophysicists, molecular biologists, geneticists,
cell biologists, physiologists, and specialists in drug design and
development, proteomics, and molecular medicine with an interest in
proteins and peptides.

Autor

Vladimir N. Uversky, PhD, is a Senior Research Professor at the Indiana University School of Medicine. He obtained his academic degrees from Moscow Institute of Physics and Technology (PhD) and the Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences. Professor Uversky has authored over 300 scientific publications and edited several books and book series on protein structure, function, folding, and misfolding. He is also an editor of several scientific journals. Sonia Longhi, PhD, is a Director of Research at the Center for the National Scientific Research (CNRS), heading the Structural Disorder and Molecular Recognition group within the AFMB laboratory. She obtained her PhD from the Università degli Studi of Milan in 1993. She has authored more than sixty scientific publications and edited a book on measles virus nucleoprotein.

Rezensionen

"With contributions from an international selection of leading researchers, this book fills an important need in a rapidly growing field. It is required reading for biochemists, biophysicists, molecular biologists, geneticists, cell biologists, physiologists, and specialists in drug design and development, proteomics, and molecular medicine with an interest in proteins and peptides." (Anticancer Research, 1 August 2010)

Produkt-Details

• Erscheint lt. Verlag: 2.2.2011
• Reihe/Serie: Wiley Series in Protein and Peptide Science
• Reihe/Serie: Wiley Series in Protein and Peptide Science
• Sprache: englisch
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Themenwelt: Naturwissenschaften ► Chemie ► Organische Chemie
• Themenwelt: Technik
• Schlagworte: Analytical Chemistry • Analytische Chemie • Biochemie • Biochemie u. Chemische Biologie • Biochemistry (Chemical Biology) • Biowissenschaften • Cell & Molecular Biology • Chemie • Chemistry • Life Sciences • Proteine • Zell- u. Molekularbiologie
• ISBN-10: 0-470-60260-0 / 0470602600
• ISBN-13: 978-0-470-60260-7 / 9780470602607