Pharmaceutical and Biomedical Applications of Capillary Electrophoresis (eBook)

S.M. Lunte, D.M. Radzik (Autoren)

eBook Download: PDF

1996 | 1. Auflage
508 Seiten
Elsevier Science (Verlag)
978-0-08-053871-6 (ISBN)

Infos

The book describes the theory and applications of Capillary Electrophoresis (CE) in the field of pharmaceutical and biomedical analysis. It is targeted towards users who are intimately involved in analytical problems, especially those which involve small samples. This book presents the technique of capillary electrophoresis from the point of view of the serious hands-on use in the field of pharmaceutical and biomedical analysis. An overview of general theory is presented to acquaint the novice with the fundamental principles. A more theoretical approach is taken in the presentation of electrokinetic chromatography. The next chapter discusses advances in column technologies, the preceding chapters having provided a foundation as to how separations occur. In the next three chapters, recognized experts in their fields present fundamentals and state-of-the-art techniques in the areas of optical, electrochemical and mass spectrometric detection. The major focus of the remaining chapters is on applications. This includes the analysis of pharmaceuticals, amino acids and peptides, macromolecules, nucleosides, nucleotides and oligonucleotides. The use of CE for analysis of small ions and separation of biological particles is also discussed. The issue of sample preparation for analysis by CE is addressed, especially as it relates to clinical analysis.

Inhalt

Front Cover 1
Pharmaceutical and Biomedical Applications of Capillary Electrophoresis 4
Copyright Page 5
Contents 6
List of Contributors 8
Introduction 10
Part One: Separations 12
Chapter 1. Principles of Capillary Electrophoresis 14
Chapter 2. Column Technologies for Capillary Electrophoresis 34
Chapter 3. Electrokinetic Chromatography 92
Part Two: Detection 190
Chapter 4. Optical Detection Strategies in Capillary Electrophoresis 192
Chapter 5. Capillary Electrophoresis/Mass Spectrometry 240
Chapter 6. Electrochemical Detection for Capillary Electrophoresis 288
Part Three: Applications 318
Chapter 7. Sample Preparation for Capillary Electrophoresis 320
Chapter 8. Capillary Electrophoresis in the Study of Amino Acids 338
Chapter 9. Capillary Electrophoresis of Proteins and Peptides 356
Chapter 10. Separation of Nucleic Acids and Oligonucleotides and Sequencing of DNA Fragments by Capillary Electrophoresis 398
Chapter 11. The Use of Capillary Electrophoresis in Pharmaceutical Development 436
Chapter 12. Capillary Electrophoresis-based Methods for in vivo Analysis: Single Cell Analysis and Microdialysis Sampling 478
Index 514

Produkt-Details

• Erscheint lt. Verlag: 13.8.1996
• Sprache: englisch
• Themenwelt: Sachbuch/Ratgeber
• Themenwelt: Medizin / Pharmazie ► Pharmazie
• Themenwelt: Naturwissenschaften ► Biologie
• Themenwelt: Naturwissenschaften ► Chemie ► Analytische Chemie
• Themenwelt: Naturwissenschaften ► Chemie ► Technische Chemie
• Themenwelt: Technik ► Umwelttechnik / Biotechnologie
• Themenwelt: Wirtschaft
• ISBN-10: 0-08-053871-1 / 0080538711
• ISBN-13: 978-0-08-053871-6 / 9780080538716