Evolutionary Approaches to Protein Design (eBook)

Frances H. Arnold (Herausgeber)

eBook Download: PDF

2000 | 1. Auflage
438 Seiten
Elsevier Science (Verlag)
978-0-08-049337-4 (ISBN)

Infos

This is the first high-quality, comprehensive overview of the field of evolutionary protein design. Topics include new protein design strategies, the structures of laboratory-evolved proteins, the evolution of non-natural enzyme functions, and the theory of laboratory evolution.

Inhalt

Cover 1
CONTENTS 6
PREFACE 10
Chapter 1. New Functions from Old Scaffolds: How Nature Reengineers Enzymes for New Functions 14
I. Introduction 14
II. Defining Protein Superfamilies 16
III. The Enolase Superfamily: A Paradigm for Understanding How Nature Evolved Enzymes for New Functions 19
IV. Studies of Other Superfamilies and Fold Classes 34
V. Conclusions 39
VI. References 39
Chapter 2. Evolution of Protein Functon by Domain Swapping 42
I. Introduction 42
II. Terminology 43
III. Evolution of Proteins in Nature by Domain Swapping 44
IV. Domain Swapping for Protein Engineering 56
V. Methodologies 72
VI. Perspective 80
References 81
Chapter 3. Rational Evolutionary Design: The Theory of In Vitro Protein Evolution 92
I. Introduction 92
II. Sequence Space and Fitness Landscapes 94
III. Modeling Directed Evolution 110
IV. Rational Evolutionary Design 151
V. Summary and Conclusions 165
References 167
Chapter 4. Temperature Adaptation of Enzymes: Lessons from Laboratory Evolution 174
I. Introduction 174
II. Influence of Temperature on Enzymes 177
III. Studies of Natural Extremophilic Enzymes 180
IV. Directed Evolution 186
V. Stability, Flexibility, and Catalytic Activity 221
VI. Why Are Proteins Marginally Stable? 229
VII. Why Are Thermophilic Enzymes Poorly Active at Low Temperature? 231
VIII. Conclusions 232
References 234
Chapter 5. Structural Analysis of Affinity Matured Antibodies and Laboratory-Evolved Enzymes 240
I. Introduction 240
II. Structural Studies of Antibody Affnity Maturation 241
III. Structural Studies of Enzyme Directed Evolution 257
IV. Conclusions 267
References 270
Chapter 6. Molecular Breeding: The Natural Approach to Protein Design 274
I. Introduction 274
II. The Need for Better Proteins 276
III. Strategies for Optimizing Proteins 277
IV. Screening Is Key 290
V. Beyond Proteins 294
VI. Concluding Remarks 298
References 299
Chapter 7. Analysis of Large Libraries of Protein Mutants Using Flow Cytometry 306
I. Introduction 306
II. Library Screening Technologies 307
III. Cell Surface Display Technologies 314
IV. Library Screening by Flow Cytometry 316
V. Concluding Remarks 324
References 324
Chapter 8. From Catalytic Asymmetric Synthesis to the Transcriptional Regulation of Genes: In Vivo and In Vitro Evolution of Proteins 330
I. Introduction and Scope of Review 330
II. Selecting and Evolving Therapeutic Human Antibodies 331
III. Directing Evolution at the Level of Chemical Mechanism: Aldolase Antibodies and Asymmetric Catalysis 344
IV. Multiple Hapten Selection: Refining the Active Site of a Catalytic Antibody by In Vitro Selection 362
V. Selection and Evolution of Novel DNA-Binding Proteins: From Principles to Applications 363
References 376
Chapter 9. In Vitro Selection and Evolution of Proteins 380
I. Introduction 380
II. The Key to In Vitro Protein Evolution: Cell-Free Translation 385
III. In Vitro Selection Strategies 387
IV. Applications of Ribosome Display 401
V. Perspectives of Directed In Vitro Evolution 411
References 412
AUTHOR INDEX 418
SUBJECT INDEX 442

Produkt-Details

• Erscheint lt. Verlag: 19.10.2000
• Sprache: englisch
• Themenwelt: Sachbuch/Ratgeber
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Themenwelt: Naturwissenschaften ► Biologie ► Evolution
• Themenwelt: Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Zellbiologie
• Themenwelt: Naturwissenschaften ► Chemie ► Organische Chemie
• Themenwelt: Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik
• Themenwelt: Technik
• ISBN-10: 0-08-049337-8 / 0080493378
• ISBN-13: 978-0-08-049337-4 / 9780080493374