Arabidopsis Protocols, 2nd Edition

Julio Salinas, Jose J. Sanchez-Serrano (Herausgeber)

Buch | Softcover

496 Seiten

2010 | Softcover reprint of hardcover 2nd ed. 2006
Humana Press Inc. (Verlag)
978-1-61737-539-2 (ISBN)

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For several decades, Arabidopsis thaliana has been the organism of choice in the laboratories of many plant geneticists, physiologists, developmental biologists, and biochemists around the world. During this time, a huge amount of knowledge has been acquired on the biology of this plant species, which has resulted in the development of molecular tools that account for much more efficient research. The significance that Arabidopsis would attain in biological research may have been difficult to foresee in the 1980s, when its use in the laboratory started. In the meantime, it has become the model plant organism, much the same way as Drosophila, Caenorhabditis, or mouse have for animal systems. Today, it is difficult to envision research at the cutting edge of plant biology without the use of Arabidopsis. Since the first edition of Arabidopsis Protocols appeared, new developments have fostered an impressive advance in plant biology that prompted us to prepare Arabidopsis Protocols, Second Edition. Completion of the Arabidopsis genome sequence offered for the first time the opportunity to have in hand all of the genetic information required for studying plant function. In addition, the development of whole systems approaches that allow global analysis of gene expression and protein and metabolite dynamics has encouraged scientists to explore new scenarios that are extending the limits of our knowledge.

Growing Arabidopsis.- Growth of Plants and Preservation of Seeds.- Prevention and Control of Pests and Diseases.- Growth of Other Species Related to Arabidopsis thaliana.- Grafting.- Synchronization, Transformation, and Cryopreservation of Suspension-Cultured Cells.- Genetic Analyses.- Genetic Analysis.- QTL Analysis.- EMS Mutagenesis of Arabidopsis.- Low-Resolution Mapping of Untagged Mutations.- Gene Identification and Cloning by Molecular Marker Mapping.- High-Throughput TILLING for Arabidopsis.- Genotyping and Mapping With High-Density Oligonucleotide Arrays.- Forward Genetic Screening of Insertional Mutants.- PCR-Based Screening for Insertional Mutants.- Cytogenetic Analyses of Arabidopsis.- Using Information From Public Arabidopsis Databases to Aid Research.- Transient and Stable Transformation.- In Planta Agrobacterium-Mediated Transformation by Vacuum Infiltration.- Transient Expression Assay by Agroinfiltration of Leaves.- Functional Analysis of Transcription Factors by Microparticle Bombardment.- Transfection Assays With Protoplasts Containing Integrated Reporter Genes.- Chloroplast Genetic Engineering Via Organogenesis or Somatic Embryogenesis.- ?-Glucuronidase as Reporter Gene.- Use of Fluorescent Proteins as Reporters.- Luciferases as Reporter Genes.- Aequorin as a Reporter Gene.- Applications of Chemical-Inducible Expression Systems in Functional Genomics and Biotechnology.- Transcriptomics.- RNA Extraction.- Target Preparation for DNA Microarray Hybridization.- Statistical Issues in Microarray Data Analysis.- Preparation and Quality Assessment of RNA From Cell-Specific Samples Obtained by Laser Microdissection.- Proteomics.- Protein Isolation and Second-Dimension Electrophoretic Separation.- Isolation of Nuclear Proteins.- Purification and Fractionationof Membranes for Proteomic Analyses.- Protein Microsequencing.- Peptide-Based Phosphoproteomics With Immobilized Metal Ion Chromatography.- Metabolomics.- Metabolite Profiling in Arabidopsis.- Hormone Profiling in Arabidopsis.

Erscheint lt. Verlag	9.12.2010
Reihe/Serie	Methods in Molecular Biology ; 323
Zusatzinfo	1 Illustrations, color; 135 Illustrations, black and white; 496 p. 136 illus., 1 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Sachbuch/Ratgeber ► Natur / Technik ► Natur / Ökologie
	Naturwissenschaften ► Biologie ► Botanik
	Naturwissenschaften ► Biologie ► Ökologie / Naturschutz
	Naturwissenschaften ► Geowissenschaften
ISBN-10	1-61737-539-X / 161737539X
ISBN-13	978-1-61737-539-2 / 9781617375392
Zustand	Neuware