Bioconjugate Techniques

Greg T. Hermanson (Autor)

Buch | Hardcover

1232 Seiten

2008 | 2nd edition
Academic Press Inc (Verlag)
9780123705013 (ISBN)

Infos

Offers information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. This book includes more than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates.

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Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.

Inhalt

PART I Bioconjugate Chemistry
1. Functional Targets
2. The Chemistry of Reactive Groups

PART II Bioconjugate Reagents
3. Zero-Length Cross-Linkers
4. Homobifunctional Cross-linkers
5. Heterobifunctional Cross-linkers
6. Trifunctional Cross-linkers
7. Dendrimers and Dendrons
8. Cleavable Reagent Systems
9. Fluorescent Probes
10. Bifunctional Chelating Agents and Radioimmunoconjugates
11. Biotinylation Reagents
12. Iodination Reagents
13. Silane Coupling Agents
14. Microparticles and Nanoparticles
15. Buckyballs, Fullerenes, and Carbon Nanotubes
16. Mass Tags and Isotope Tags
17. Chemoselective Ligation; Bioorthogonal Reagents
18. Discrete PEG Compounds

PART III Bioconjugate Applications
19. Preparation of Hapten - Carrier Immunogen Conjugates
20. Antibody Modification and Conjugation
21. Immunotoxin Conjugation Techniques
22. Preparation of Liposome Conjugates and Derivatives
23. Avidin - Biotin Systems
24. Preparation of Colloidal-Gold-Labeled Proteins
25. Modification with Synthetic Polymers
26. Enzyme Modification and Conjugation
27. Nucleic Acid and Oligonucleotide Modification and Conjugation
28. Bioconjugation for the Study of Protein Interactions

Produkt-Details

• Erscheint lt. Verlag: 3.6.2008
• Verlagsort: San Diego
• Sprache: englisch
• Maße: 191 x 235 mm
• Gewicht: 1810 g
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• ISBN-13: 9780123705013 / 9780123705013
• Zustand: Neuware