Whole Genome Amplification

Methods and Protocols

Yafeng Ma (Herausgeber)

Buch | Hardcover

227 Seiten

2026
Humana (Verlag)
9781071650592 (ISBN)

Infos

This volume focuses on the broad application of whole genome amplification (WGA) in cancers, preimplantation genetic testing, and forensic science. The chapters in this book cover topics such as upstream challenges (sample preparation, quality control) and downstream applications (next-generation sequencing, SNP detection). This book also explores how WGA’s have impacted fields like cancer genomics (detecting subclonal mutations), prenatal testing (non-invasive aneuploidy screening), and microbiome research (amplifying low-biomass communities). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Whole Genome Amplification: Methods and Protocols is a valuable resource for all researchers who are looking to learn more about this important and advancing field.

Chapter 13 is available Open Access under a Creative Commons Attribution 4.0. International License via link.springer.com.


 

Inhalt

Whole-Genome Amplification for Circulating Tumor Cells.- Whole Genome Amplification on Single Circulating Trophoblast Cell.- A Tailing Method of Quality Control on MALBAC-Based Whole-Genome Amplification Products.- Micromanipulation and Multiple-Strand Displacement Amplification for Forensic DNA Profiling of Single Sperm Cells.- Whole-Genome Amplification of Single Circulating Tumor Cells from Mice Xenografts.- An Integrated Preimplantation Genetic Testing Approach with MALBAC-Based Whole-Genome Sequencing.- Handmade Biopsies and Whole Genome Amplification for Genotyping and Genomic Selection of Bovine Preimplantation Embryos.- Accelerating Genomics: Innovations in Analyzing Limited and Rare Samples.- Sweet Sorghum Microspores’ Fractionalization, Individual Isolation, and Whole Genome Amplification.- Assessing Amplification Quality and Bias in MDA Methods through Comparative Analysis of Short-Read Sequencing.- Sequencing RSV Whole Genome Using a Long Amplicon-Based Method with Oxford Nanopore Technologies.- Laser Microdissection and Near Single-Cell Whole Genome Amplification.- Whole Genome Amplification of Microbial DNA from Host-Depleted Clinical Samples.- Whole Genomic Amplification for Detecting Single-Gene Variants Causing Inherited Disease.- Single-Cell Low-Pass Whole Genome Sequencing of Circulating Tumor Cells.- Single Oocyst Selection and Whole Genome Amplification of Cryptosporidium parvum.

Produkt-Details

• Erscheinungsdatum: 29.11.2025
• Reihe/Serie: Methods in Molecular Biology
• Zusatzinfo: 42 Illustrations, color; 6 Illustrations, black and white
• Sprache: englisch
• Maße: 178 x 254 mm
• Themenwelt: Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
• Schlagworte: archived miracidia • DNA quality control • SARS-Cov-2 virus • single circulating tumour cell • single droplet MDA
• ISBN-13: 9781071650592 / 9781071650592
• Zustand: Neuware