Immune Mediators in Cancer
Humana (Verlag)
978-1-0716-4900-8 (ISBN)
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Authoritative and cutting-edge, Immune Mediators in Cancer: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
A Useful Guide for Analysis of Biomarkers in Cancer by Fluorochrome (Luminex) Technique.- Detection of Multi-analytes Using Luminex Multiplex Bead Immunoassay.- Flow Cytometry Analysis of IFNγ-induced PD-L1 Cell Surface Expression in Ovarian Cancer Cells.- Evaluating Nuclear Levels of PD-L1 in Ovarian Cancer Cells by Western Blotting.- Combined Single-Cell Measurement of Cytokine mRNA and Protein in Immune Cells.- A Beginner’s Guide to Immunohistochemistry Technique for Paraffin Sections.- Immunohistochemistry and Immunofluorescence Approaches in Cancer Research Using Paraffin-Embedded Tissue Sections.- Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence.- Masson’s Trichrome Staining Technique to Evaluate Tissue Fibrosis.- 3D Spheroid Model: A Methodological Approach for Advancing Cancer and Immunology Research.- Analysis of IFNγ-Induced Invasion of Ovarian Cancer Cells using 3D Spheroids.- Use of Biomimetic Hydrogels and 3D Cancer Models for Biochemical and Cell Biological Assays.- Detection of Ferroptosis by Liperfluo and BODIPY™ 581/591 C11.- Methods for Measuring TNFα-induced Autophagy in Cancer Cells.- Evaluation of Metabolic Characteristics of Cancer Cells .- Assessing Intracellular Metabolism of Immune Cells in Situ in Live Zebrafish Larvae by Autofluorescence Lifetime Imaging Microscopy of NAD(P)H and FAD.- Macrophage Enhancement of Tumor Cell Extravasation and Intravasation In Vitro.- Differentiation and Polarization of THP-1 Cells into M1 and M2 Macrophages for Cancer Research.- Analysis of PD-L1 Transcriptional Activity by Chromatin Immunoprecipitation.- Semi-quantitative RT-PCR Assay for the Analysis of Alternative Splicing of Interleukin Genes.- Proximity Ligation Assay to Detect Protein-Protein Interaction in Cancer Cells.- Measuring Chimeric Antigen Receptor T Cells (CAR T Cells) Activation by Coupling Intracellular Cytokine Staining with Flow Cytometry.- Fluorescence Lifetime Imaging Application to Probe GTPase Activation in Macrophage Cell Line, using the Time-domain FastFLIM Modality.- Optimized Whole-Mount X-gal Staining to Detect Hedgehog Signaling Activity in Basal Cell Carcinoma: A Platform for Spatial Integration with Immune Analysis.- Targeting Fibrosis in Arthritis using mRNA Vaccine-Based Approach.- Engineering Mutation Clones in Mammalian Cells with CRISPR/Cas9.- Generation of Knockout Cell Lines Using CRISPR/Cas9-Based Genome Editing.- High-Throughput RNA Interference Screen Targeting Synthetic-Lethal Gain-of-Function of Oncogenic Mutant TP53 in Triple-Negative Breast Cancer.- Rapid Exploratory Data Visualization for Cancer Genomics.- Evolutionary Insights from Sequence Analysis of Interleukin 17A (IL17A).- Methods to Identify and Validate Therapeutic Targets against Cancer-Associated Fibroblasts in Head and Neck Squamous Cell Carcinoma.- Bioinformatics Analysis of Bc3, IL-8 and PD-L1 Gene Coexpression in Glioblastoma and Pan-cancer using Xena Platform.- Single-Cell 3’ mRNA Sequencing with 10x Chromium Gel Beads-in-Emulsion (GEM) Kits.- The Experimental Procedure of Somatic Hypermutation Analysis in Splenic B Cells During Primary Response.
| Erscheinungsdatum | 29.11.2025 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology |
| Zusatzinfo | 93 Illustrations, color; 18 Illustrations, black and white |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie |
| Medizin / Pharmazie ► Studium | |
| Naturwissenschaften ► Biologie | |
| Schlagworte | Bioassays • Cancer cells • cancer diagnosis • Cytokines and chemokines • immunoassays |
| ISBN-10 | 1-0716-4900-0 / 1071649000 |
| ISBN-13 | 978-1-0716-4900-8 / 9781071649008 |
| Zustand | Neuware |
| Informationen gemäß Produktsicherheitsverordnung (GPSR) | |
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