Functional and structural studies of an atypical fungal endoglucanase

Marina Paglione Ramia (Autor)

Buch | Softcover

96 Seiten

2025
Our Knowledge Publishing (Verlag)
978-620-8-63347-9 (ISBN)

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The importance of studying cellulases is not limited to the acquisition of scientific knowledge, but also to the great biotechnological potential they represent. This is due to the fact that cellulose is the most abundant molecule present in nature and provides a wide range of sustainable products and processes. Many cellulase families have already been well characterized, while others remain unknown. Among the latter, family 45 of glycoside hydrolases is the least characterized family of fungal cellulases, both structurally and functionally. In this book we report the crystallographic structure of the endoglucanase V enzyme from a microorganism called Phanerochaete chrysosporium (PcCel45A). We also report the structure of this enzyme with a ligand (cellobiose). In order to study its function, we carried out studies on the enzyme's catalytic site, using site-directed mutagenesis in the main residues of the active site. This enzyme showed a structure and mechanism of action that was totally different from the other enzymes in the family.

Marina Paglione Ramia graduated in Biological Sciences from the Federal University of São Carlos (2011). During this period she worked with nuclear receptors, focusing on their structural characterization. She completed her PhD at the São Carlos Institute of Physics (USP), working on the study of cellulolytic enzymes.

Erscheinungsdatum	09.02.2025
Sprache	englisch
Maße	152 x 229 mm
Gewicht	141 g
Themenwelt	Naturwissenschaften ► Chemie ► Physikalische Chemie
Schlagworte	Cellulase • crystallography • endoglucanase
ISBN-10	620-8-63347-8 / 6208633478
ISBN-13	978-620-8-63347-9 / 9786208633479
Zustand	Neuware