Histones

Methods and Protocols

Luc Gaudreau, Benoit Guillemette (Herausgeber)

Buch | Hardcover

292 Seiten

2025 | Second Edition 2025
Springer-Verlag New York Inc.
9781071644850 (ISBN)

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This second edition provides new and updated chapters on histones for molecular biologists, biochemists and geneticists. Chapters cover protein complexes that modify chromatin either by adding or removing post-translational modifications, or by exchanging histone variants within the nucleosome. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Histones: Methods and Protocols, Second Edition aims to be comprehensive guide for researchers in the field.

In vitro assembly of nucleosomes for binding/remodeling assays.- Ruthenium-catalyzed one-pot peptide ligation.- A method for H2A.Z/H2B dimer exchange within nucleosomes in vitro.- Automated sample preparation for the unbiased analysis of histone post-translational modifications via mass spectrometry.- Detection of γ -H2A.X for Rapid Assessment of Genotoxic Agents-Induced Double-Stranded DNA breaks by Immunofluorescence.- High Resolution Cryo-EM Analyses of Nucleosomes.- Correlative Electron spectroscopic Imaging (ESI) and electron tomography of chromatin.- Psoralen crosslinking-Chromatin endogenous cleavage assay to examine histone DNA interactions of active and inactive rRNA genes.- Genome-wide nucleosome mapping by H3Q85C-directed chemical cleavage in Saccharomyces cerevisiae.- Efficient in-house coupling of sample and library preparation for ChIP-seq of histone modifications in complex plant tissues.- Chromatin immunoprecipitation assays of histone modifications in Daphnia magna.- Single-cell epigenomic profiling with high-throughput droplet scChIP-seq.- Determining the Substrate Specificity of Lysine Methyltransferases.- Identification of chromatin readers using a peptide pull-down and mass spectrometry integrated approach.- Efficient, Conditional Depletion of Nuclear Proteins in Saccharomyces cerevisiae.- RNA Immunoprecipitation (RIP) from Purified Nuclei in Cells.

Erscheinungsdatum	24.04.2025
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	38 Illustrations, color; 14 Illustrations, black and white; XI, 292 p. 52 illus., 38 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
Themenwelt	Naturwissenschaften ► Chemie ► Organische Chemie
Schlagworte	Cas9 • c elegans • electron microscopy • epigenetic model organism • Mass Spectrometry
ISBN-13	9781071644850 / 9781071644850
Zustand	Neuware