Agrobacterium

Methods and Protocols

Buch | Hardcover

200 Seiten

2025
Humana (Verlag)
978-1-0716-4449-2 (ISBN)

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This volume details Agrobacterium strain methods, such as plant genotype, transient and stable transformation of plants for gene overexpression, silencing, transplastomic and editing. Transformation protocols are focused mainly in plants with economic importance such as cereals and fruit trees including diploids and polyploids, and in non-plant species such fungi and algae. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Agrobacterium: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Preparation of chemocompetent Agrobacterium tumefaciens cells.- Molecular analysis of Agrobacterium tumefaciens transformed strains with commercially available Plasmid DNA Miniprep kits.- Using Agrobacterium tumefaciens to assemble multi-step metabolic pathways in Nicotiana benthamiana.- Verifying plasmid constructs via transient Agrobacterium tumefaciens-mediated plant transformation in Nicotiana benthamiana.- Vacuum-Assisted Transient Agrobacterium tumefaciens-Mediated Transformation for plant molecular analysis.- Agrobacterium tumefaciens-mediated plant transformation and gene editing in rice.- Agrobacterium-Mediated Transformation for Commercial Wheat Varieties.- Agrobacterium-mediated transformation of Soybean (Glycine max L.) using split-cotyledonary explant.- Genetic transformation of Lettuce (Lactuca sativa) using Agrobacterium tumefaciens.- Agrobacterium tumefaciens-mediated genetic transformation of Nigella damascene.- Agrobacterium tumefaciens mediated stable cotyledon transformation of Solanum lycopersicum var Micro-Tom.- Agrobacterium-mediated transformation for gene editing tomato elite breeding lines.- Agrobacterium tumefaciens-mediated stable transformation of Malus domestica (Apple) cv Fuji RakuRaku and Royal Gala.- Agrobacterium tumefaciens-Mediated Transformation of Grape (Vitis vinífera sp).- Agrobacterium-Mediated Stable Transformation of Actinidia deliciosa (Kiwi).- Transformation of mature apricot (Prunus armeniaca L.) hypocotyl slices.- Agrobacterium-Mediated Transformation of Prunus persica (Peach) Immature Cotyledons.

Erscheinungsdatum	14.05.2025
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	24 Illustrations, color; 7 Illustrations, black and white
Verlagsort	New York
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	gene expression • plant genotypification • rizogenes-mediated transformation • Transient Agrobacterium • tumefaciens
ISBN-10	1-0716-4449-1 / 1071644491
ISBN-13	978-1-0716-4449-2 / 9781071644492
Zustand	Neuware