Epigenetics Methods

Buch | Softcover

746 Seiten

2025 | 2nd edition
Academic Press Inc (Verlag)
978-0-443-26759-8 (ISBN)

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Epigenetics Methods, Second Edition offers an extensive overview of the tools that enhance translational epigenetics studies. This edition includes updated and revised chapters, along with newly added topics such as MALDI-TOF-MS for analyzing DNA methylation and the RIP assay for lncRNA binding. Specialists provide step-by-step guidance on methods used to study various epigenetic mechanisms. The book covers both fundamental and advanced techniques, making it an essential resource for researchers in this dynamic field. In addition to DNA methylation and histone modification techniques, the book also explores chromatin evaluation, enzyme analyses, and non-coding RNAs as epigenetic modulators.

New chapters discuss recent advancements like single-cell epigenomics, epigenetic editing, and computational epigenetics. The volume addresses reproducibility issues and offers consensus-driven methods to unify approaches across experiments and labs. This edition is a vital reference for understanding and employing cutting-edge epigenetics methods.

Dr. Tollefsbol is a Distinguished Professor at the University of Alabama at Birmingham. He holds doctorates in molecular biology and osteopathic medicine, trained with National Academy of Science members and has over 200 peer-reviewed publications. Reports highlighting the translatability to the lay public of the discoveries made in his laboratory have appeared in international media representing >50 million readers. Dr. Tollefsbol has been featured as an Investigator in the Spotlight by the NIH as well as a Scientist in the Spotlight by ScienceNow. He is an Associate Editor for Frontiers in Genetics, a contributor to Lewin’s GENES classic textbook and Lead Editor for Elsevier’s Translational Epigenetics Series. Dr. Tollefsbol has been named a “Highly Ranked Scholar worldwide (ScholarGPS). He has been invited for numerous endowed keynote presentations, his research has been highlighted in eScience News and ScienceDaily and he has published 20 scholarly books on topics related to his research.

Section 1: Overview
1. An Overview of Epigenetics Methods

Section 2: DNA Methylation Methods: Gene- or Locus-specific Methylation Analyses
2. Principles of Bisulfite Conversion of DNA and Methylation-specific PCR (MSP) in Biological Research
3. Multiplex Methylation Specific Droplet Digital PCR (MS-ddPCR) for Detection and of Rare Methylated Alleles

Section 3: DNA Methylation Methods: Global DNA Methylation and Methylomic Analyses
4. Methods for Detection of DNA Methylation in Transposable Elements
5. The Illumina Infinium Methylation Assay for Genome-wide Methylation Analyses
6. Methylated DNA Immunoprecipitation Sequencing: Methodological Principles and Applications
7. Multiplex Analyses Using Methylation Sensitive Restriction Enzyme coupled qPCR and NGS
8. Advances in Whole Genome Methylomic Sequencing

Section 4: DNA Methylation Methods: Additional Technologies
9. Analyzing DNA Methyltransferase Enzymatic Activity: Basic Methods and Applications
10. Technologies and Applications for the Assessment of 5-Hydroxymethylcytosine

Section 5: Histone Modification Methods
11. The Chromatin Immunoprecipitation (ChIP) Assay and ChIP-qPCR
12. CUT&RUN and CUT&Tag for histone modification and transcription factor binding analyses
13. Deep Sequencing for Histone Modifications Employing ChIP-seq Analyses
14. ChIP in Series: Sequential ChIP
15. The ChIP-exo Method to Study DNA-protein interactions and Chromatin Organization at Near-Nucleotide Resolution
16. ATAC-seq Method Applied to Embryonic Germ Cells and Neural Stem Cells from Mouse: Practical
17. Combinatorial ChIP and Bisulfite Sequencing (BisChIP-seq)

Section 6: Epigenetic Methods for Evaluating Chromatin Higher Order
18. 3D DNA FISH for Analyses of Nuclear Chromatin Architecture

Section 7: Enzyme Analyses of Histone Modifications
19. Assessment of Histone-Modifying Enzymes Involved in Epigenetic Gene Regulation

Section 8: Assessment of Non-Coding RNA as an Epigenetic Modulator
20. Detection and Quantification of MicroRNAs (miRNAs) and High Throughput miRNAs Profiling
21. RIP-seq in Epigenetic Analyses
22. Chromatin Isolation by RNA Purification (ChIRP) and Its Applications

Section 9: Additional Epigenetics Methods
23. Single-Cell Epigenomics: Methods and Translation
24. CRISPR/Cas9 Technologies in Epigenetics Research
25. Epigenome-Wide Association Study (EWAS) Methods and Applications
26. Systems Biology Methods to Study the Epigenome and Metabolic- epigenetic Interactions
27. Forensic Epigenetics Methods and Applications

Section 10: Future Directions in Epigenetics Methodology
28. Advances in Epigenetic Techniques to Study Development and Diseases

Erscheinungsdatum	02.09.2025
Reihe/Serie	Translational Epigenetics
Verlagsort	San Diego
Sprache	englisch
Maße	191 x 235 mm
Gewicht	450 g
Themenwelt	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
ISBN-10	0-443-26759-6 / 0443267596
ISBN-13	978-0-443-26759-8 / 9780443267598
Zustand	Neuware