Molecular Motors

Methods and Protocols

Christophe Lavelle, Antoine Le Gall (Herausgeber)

Buch | Hardcover

358 Seiten

2024 | Third Edition 2025
Humana (Verlag)
978-1-0716-4279-5 (ISBN)

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This fully updated volume presents a diverse collection of protocols and methodologies for studying various molecular motors. The book explores the dynamics of the bacterial flagellar motor, the mechanisms of ATP synthase, the assembly and function of cytoskeletal motors, and the role of nucleic acid motors in cellular processes, while placing a strong emphasis on state-of-the-art techniques, highlighting innovative methods such as single-molecule visualization and high-resolution tracking, sophisticated biochemical assays, bioengineering applications, as well as computational modeling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Molecular Motors: Methods and Protocols, Third Edition serves as an ideal guide to enable researchers to probe the mechanisms of molecular motors with unprecedented precision and detail.

Cellular and Nuclear Forces: An Overview.- Measuring Bacterial Flagellar Motor Dynamics via a Bead Assay.- Purification and Reconstitution of Ilyobacter tartaricus ATP Synthase.- Motor Function of the Two-Component EEA1-Rab5 Revealed by dcFCCS.- Tracking Single Kinesin in Live Cells Using MINFLUX.- Multicolor Tracking of Molecular Motors at Nanometer Resolution.- Engineering Dynein Motors to Move on DNA Nanotube Tracks.- Engineering Synthetic Myosin Filaments Using DNA Nanotubes.- Expression, Purification, and In Vitro Analysis of Myosin.- Single-Molecule Visualization of BLM-DNA2-Mediated DNA End Resection Using DNA Curtains.- Isolation and Analysis of Rare Enzymatic Events with Multiplex Flow Magnetic Tweezers.- Homologous Recombination and DNA Intermediates Analyzed by Electron Microscopy.- Electrophoretic Analysis of DNA Supercoiling Activities.- Remodeling of Individual Nucleosomes in Nucleosome Arrays.- Methods for Studying Motor-Driven Viral DNA Packaging in Bacteriophages phi29, Lambda, and T4 via Single DNA Molecule Manipulation and Rapid Solution Exchange.- Preparation of a Substrate for Studying Nucleosomes on Single Molecules of DNA.- Assessing the Effect of Chromatin-Binding Enzymes on Condensed Chromatin with Optical Tweezers.

Erscheinungsdatum	21.12.2024
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	65 Illustrations, color; 8 Illustrations, black and white
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Themenwelt	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	ATP synthase • Bacterial flagellar motor • Cytoskeletal motors • Nanomachines • Nucleic acid motors • Nucleotide hydrolysis
ISBN-10	1-0716-4279-0 / 1071642790
ISBN-13	978-1-0716-4279-5 / 9781071642795
Zustand	Neuware