Meiosis

Methods and Protocols

Buch | Hardcover

292 Seiten

2024 | 2024 ed.
Springer-Verlag New York Inc.
9781071639054 (ISBN)

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This volume details protocols on meiosis, covering the latest technological and methodological advances in this research field. Chapters guide readers through methods on genomics, biochemistry, super-resolution microscopy, traditional genetics, cytological methods, as well as machine learning and in silico modelling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Meiosis: Methods and Protocols aims to ensure successful results in further studies of this vital field.

CC-seq Nucleotide Resolution Mapping of Spo11 DNA Double Strand Breaks in S cerevisiae Cells.- SPO Seq An Accessible Method for Efficient Evaluation of Spo11 Catalytic Activity and Profiling Meiotic DSB Hotspots in Saccharomyces cerevisiae.- Hi-C2B: Optimised Detection of Chromosomal Contacts within Synchronised Meiotic S cerevisiae Cells.- Efficient Enrichment of Synchronized Mouse Spermatocytes Suitable for Genome-Wide Analysis.- A Method to Study the Meiotic Recombination Map in Human Preimplantation Blastocysts.- Generation of Meiotic Mouse Models Using CRISPR/Cas9 Technology.- Isolation of Homogenous Sub-Populations of Spermatocytes from Mouse Testis.- Analysis of Meiotic Progression by ex vivo Culture of Mouse Embryonic Ovaries.- The Organotypic Culture of Mouse Seminiferous Tubules as a Reliable Methodology for the Study of Meiosis in vitro.- Dynamic Live Cell Imaging of Budding Yeast Meiosis.- Visualization and Quantification of Rapid Chromosome Movements at Early Stages of Mouse Meiosis.- Culture of the Intact Postnatal Naked Mole-rat Ovary From Meiotic Prophase to Single Cell RNASeq.- Temporal Analysis of DSB Repair Outcome in C elegans Meiosis.- Rapamycin-induced translocation of meiotic nuclear proteins in Saccharomyces cerevisiae.- Synapsis A Bioconductor Package to Automate the Analysis of Meiotic Double-Strand Break Repair and Crossover Formation.- Automated Quantification of Meiotic Recombination Foci Position and Intensity.- Computational Tools for the Analysis of Meiotic Prophase I Images.- Identifying Chromosome Movement Patterns During Meiosis Using ChroMo.

Erscheinungsdatum	13.08.2024
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	6 Illustrations, black and white; XII, 292 p. 6 illus. With online files/update.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	Cryo-EM • DNA replications • mitotic prophase • proteinaceous structure • recombination machinery
ISBN-13	9781071639054 / 9781071639054
Zustand	Neuware