Protocols for Gene Transfer in Neuroscience

Towards Gene Therapy of Neurological Disorders

Pedro Lowenstein, Lynn W. Enquist (Herausgeber)

Buch | Softcover

446 Seiten

1996
John Wiley & Sons Ltd (Verlag)
978-0-471-95766-9 (ISBN)

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This is a study of the latest techniques used by gene delivery laboratories. The methodology under discussion includes physical transfection techniques, various viral vectors, and retrovirally-transduced cell and bone marrow transplantation. Several variations of a single protocol are given, thus demonstrating how different labs have solved a common problem in different ways. The protocols are also indexed and cross-referenced so that an investigator can find all the details required for a particular problem.

PROTOCOLS FOR GENE DELIVERY TO CULTURED CELLS USING PHYSICAL METHODS: Gene Therapy into Post-Mitotic Neurons in Culture by Microinjection; PROTOCOLS FOR GENE DELIVERY USING RNA VIRUSES: Semliki Forest Virus Vectors for Gene Transfer; PROTOCOLS FOR GENE DELIVERY USING ADENOVIRUSES AND ADENO-ASSOCIATED VIRUSES: Adeno-Associated Virus Vectors for Gene Transfer; PROTOCOLS FOR GENE DELIVERY USING HERPES SIMPLEX VIRUS 1 VECTORS: Transcriptional Regulation of Herpes Simplex Virus Gene Expression: Implications for our Understanding of Latency; PROTOCOLS FOR GENE DELIVERY USING CELLS: Murine Mucopolysaccharides Type VII: A Model System for Somatic Gene Therapy of the Central Nervous System; PROTOCOLS FOR IN VIVO APPLICATION OF GENE TRANSFER METHODS: Replication-Competent Viral Therapy of Tumours in the Central Nervous System; PROSPECTS AND SHORTCOMINGS OF NEUROLOGICAL GENE THERAPY.

Erscheint lt. Verlag	26.4.1996
Zusatzinfo	index
Verlagsort	Chichester
Sprache	englisch
Maße	210 x 245 mm
Gewicht	1030 g
Themenwelt	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
	Naturwissenschaften ► Biologie ► Humanbiologie
	Naturwissenschaften ► Biologie ► Zoologie
	Technik ► Umwelttechnik / Biotechnologie
ISBN-10	0-471-95766-6 / 0471957666
ISBN-13	978-0-471-95766-9 / 9780471957669
Zustand	Neuware