Biochemical Pathways and Environmental Responses in Plants: Part C

Buch | Hardcover

324 Seiten

2023
Academic Press Inc (Verlag)
978-0-443-13197-4 (ISBN)

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Biochemical Pathways and Environmental Responses in Plants: Part C, Volume 683 in the Methods in Enzymology series, highlights advances in the field. with this new volume presenting chapters on topics including Preparation of hydroxycinnamoyl-CoA thioesters using recombinant 4-coumarate:coenzyme A ligase (4CL) for characterization of BAHD acyltransferases, Near-real time determination of BAHD acyl-coA transferase reaction rates, Bioinformatic tools for protein structure prediction and for molecular docking applied to enzyme active site analysis, Computational and biochemical methods to measure the activity of carboxysomes and protein organelles in vivo, Dirigent Proteins Family Function and Structure, Three-in-One Method for High-throughput plant multi-omics, amongst other timely topics.

Additional sections cover Analysis of isoprenyl-phosphates by liquid chromatography-mass spectrometry, Lignin/Lignan Biosynthesis Structure/Function, Comparison of TLC, HPLC, and direct infusion mass spectrometry methods for identification and quantification of diacylglycerol molecular species, Plant sphingolipid analysis, RNA-seq analysis of alternative pre-mRNA splicing mediated by photoreceptors in Physcomitrium patens, and much more.

Prof. Jez received his B.S. in biochemistry from Penn State University (1992), a Ph.D. in biochemistry & molecular biophysics from the University of Pennsylvania (1998), and was an NIH-NRSA postdoctoral fellow at the Salk Institute for Biological Studies (1998-2001). After working as a research scientist at Kosan Biosciences (2001-2002), he started his research group at the Donald Danforth Plant Science Center and moved to the Department of Biology at Washington University in 2008. He has authored more than 160 papers and received a Presidential Early Career Award for Scientists and Engineers (2005), the Phytochemical Society of North America's Arthur Neish Young Investigator Award (2007), and a Fulbright Senior Specialist Award (2012) and was named as Howard Hughes Medical Institute Professor in 2014. Research in the Jez lab seeks to understand how environmental changes re-model biochemical pathways in plants at the molecular, cellular, and organism levels with the aim of engineering these systems to address agricultural and environmental problems. Current work in the lab employs a combination of structural biology, protein chemistry, and plant biology. He also launched the Biotech Explorers Program, which aims to introduce undergraduates to how teams in science can tackle real world problems using cross-disciplinary approaches.

Protein
1. Preparation of hydroxycinnamoyl-CoA thioesters using recombinant 4-coumarate:coenzyme A ligase (4CL) for characterization of BAHD acyltransferases
Michael Sullivan
2. Near-real time determination of BAHD acyl-coA transferase reaction rates
Michael Sullivan
3. Bioinformatic tools for protein structure prediction and for molecular docking applied to enzyme active site analysis
Michael Sullivan & Amanda Fanelli
4. Computational and biochemical methods to measure the activity of carboxysomes and protein organelles in vivo
Jeffrey Cameron
5. Dirigent Proteins Family Function and Structure
Norman Lewis

Metabolite
6. Three-in-One Method for High-throughput plant multi-omics
Sixue Chen
7. Analysis of isoprenyl-phosphates by liquid chromatography-mass spectrometry
Peter Doermann
8. Lignin/Lignan Biosynthesis Structure/Function
Norman Lewis
9. Comparsion of TLC, HPLC, and direct infusion mass spectrometry methods for identification and quantification of diacylglycerol molecular species
Phil Bates
10. Plant sphingolipid analysis
Jennifer Markam

Environment
11. RNA-seq analysis of alternative pre-mRNA splicing mediated by photoreceptors in Physcomitrium patens
Shih-Long Tu
12. Monitoring lipid-protein interactions in planta using Forster resonance energy transfer
Sam Wang
13. Acetylene reduction assay for nitrogenase activity under salinity stress
Senthil Subramanian and Liping Gu
14. Assessing simultaneious reversible cysteine oxidation and global abundance in plant tissues
Leslie Hicks
15. High-throughput phenotyping and the use of computer vision to unravel novel phenotypes associated with plant nutrition
David Mendoza-Cozatl
16. Using Hyper as a Molecular Probe to visualize hydrogen peroxide in living cells
Luis Cardenas
17. A new probe DC_-Rho1 for visualizing protein sulfenylation in living plant cells
Luis Cardenas

Erscheinungsdatum	28.04.2023
Reihe/Serie	Methods in Enzymology
Verlagsort	San Diego
Sprache	englisch
Maße	152 x 229 mm
Gewicht	450 g
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Botanik
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
ISBN-10	0-443-13197-X / 044313197X
ISBN-13	978-0-443-13197-4 / 9780443131974
Zustand	Neuware