Isolation: The key to good bacterial identification

Performing bacterial cultures in the laboratory: importance of obtaining isolated colonies

Kevin Mael Sintondji (Autor)

Buch | Softcover

52 Seiten

2021
Our Knowledge Publishing (Verlag)
9786204275208 (ISBN)

Infos

Samples such as pus and urine are usually polymicrobial. It is therefore important to separate the bacteria responsible for the pathology from the others, otherwise a mixture of germs is cultivated, which complicates identification. This technique for separating bacteria is called isolation and can be performed using at least two methods (French or three quadrants and English or four quadrants), each of which has its advantages and limitations. The general objective of this study was to identify each germ present in an inoculum based on the various representative colonies obtained. The results of this study show that both isolation methods allow to obtain representative colonies of each germ but the English method is the most efficient because it allows a homogeneous distribution of colonies. Thus the identification of each representative colony is easily done whereas the identification of confluent colonies is difficult to resolve and erroneous. Key words : Bacterial culture, laboratory, isolated colonies

Autor

Sintondji, Kevin MaelBiologist by training, I was born on January 19, 1998 of Beninese parents. Passionate about scientific research, I am part of a research unit in microbiology and pharmacology whose main members are authors of several publications in international journals. I am currently pursuing my master studies.

Produkt-Details

• Erscheinungsdatum: 01.07.2022
• Sprache: englisch
• Maße: 150 x 220 mm
• Gewicht: 96 g
• Themenwelt: Naturwissenschaften ► Biologie ► Allgemeines / Lexika
• Themenwelt: Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
• Schlagworte: bacterial culture • isolated colonies. • Laboratory
• ISBN-13: 9786204275208 / 9786204275208
• Zustand: Neuware