Short Protocols in Molecular Biology

A Compendium of Methods from Current Protocols in Molecular Biology

Frederick M. Ausubel, Roger Brent, Robert E. Kingston, David D. Moore, J.G. Seidman (Herausgeber)

Buch | Softcover

1512 Seiten

2002 | 5th Revised edition
John Wiley & Sons Inc (Verlag)
978-0-471-25092-0 (ISBN)

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Expanded to 2 volumes, Short Protocols in Molecular Biology, Fifth Edition, provides condensed descriptions of more than 700 methods compiled from Current Protocols in Molecular Biology. Includes new chapters on chromatin assembly and analysis, nucleic acid arrays, generation and use of combinatorial libraries, discovery and analysis of differentially expressed genes in single cells and cell populations. The book is specifically designed to provide quick access to step-by-step instructions for the essential methods used in every major area of molecular biological research Short Protocols in Molecular Biology, Fifth Edition is an authoritative and indispensable guide for all life scientists, researchers, and students at the graduate and advanced undergraduate level.

VOLUME 1 Chapter 1: Eschericia Coli,:Plasmids, and Bacteriophages Chapter 2: Preparation and Analysis of DNA Chapter 3: Enzymatic Manipulation of DNA AND RNA Chapter 4: Preparation and Analysis of RNA Chapter 5: Construction of Recombinant DNA Libraries Chapter 6: Screening of Recombinant Libraries Chapter 7: DNA Sequencing Chapter 8: Mutagenesis of Cloned DNA Chapter 9: Introduction of DNA into Mammalian Cells Chapter 10: Analysis of Proteins Chapter 11: Immunology VOLUME 2 Chapter 12: DNA-Protein Interactions Chapter 13: Saccharomyces Cerevisiae Chapter 14: In Situ Hybridization and Immunohistochemistry Chapter 15: The Polymerase Chain Reaction Chapter 16: Protein Expression Chapter 17: Analysis of Protein Phosphorylation Chapter 18: Informatics for Molecular Biologists Chapter 19: Analysis of Protein Interactions Chapter 20: Chromatin Assembly and Analysis Chapter 21: Nucleic Acid Arrays Chapter 22: Generation and Use of Combinatorial Libraries Chapter 23: Discovery and Analysis of Differentially Expressed Genes in Single Cells and Cell Populations Appendices

Erscheint lt. Verlag	13.12.2002
Zusatzinfo	illustrations
Verlagsort	New York
Sprache	englisch
Maße	213 x 278 mm
Gewicht	3472 g
Einbandart	Paperback
Themenwelt	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
ISBN-10	0-471-25092-9 / 0471250929
ISBN-13	978-0-471-25092-0 / 9780471250920
Zustand	Neuware