Type 2 Diabetes

Methods and Protocols

Buch | Hardcover

300 Seiten

2019 | Second Edition 2020
Humana Press Inc. (Verlag)
978-1-4939-9880-7 (ISBN)

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This second edition volume expands on the previous editions with updates on the commonly used techniques and analysis used by clinicians and scientists to study diabetes. The chapters in this book cover topics such as nutritional models of type 2 diabetes mellitus; the isolation and purification of rodent pancreatic islets of Langerhans; nitric oxide and redox state measurements in pancreatic beta cells; and primary adipocytes as a model for insulin sensitivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Type 2 Diabetes: Methods and Protocols, Second Edition is a valuable resource for all researchers who are interested in learning more about this important and evolving field.

Sixty Years of Drug Discovery for Type 2 Diabetes: Where Are We Now?.- Practical Considerations for In Vivo Studies.- Nutritional Models of Type 2 Diabetes Mellitus.- Cheminformatics in the Identification of Drug Classes for the Treatment of Type 2 Diabetes.- Whole Exome Sequencing (WES) for Illumina HISeq using Solution-Based Capture.- Gene Expression Mining in Type 2 Diabetes Research.- Pathways Enrichment Analysis of Gene Expression Data in Type 2 Diabetes.- Diagnostic Genetic Testing for Monogenic Diabetes and Congenital Hyperinsulinemia.- The Isolation and Purification of Rodent Pancreatic Islets of Langerhans.- Characterization of Islet Leukocyte Populations in Human and Murine Islets by Flow Cytometry.- Analysis of Histone Modifications in Rodent Pancreatic Islets by Native Chromatin Immunoprecipitation.- Quantification of Pancreatic Islets: Using Image Analysis Tools.- The Measurement of Calcium Signaling In BetaCell Lines using Epifluorescence and Confocal Microscopy.- Nitric Oxide and Redox State Measurements in Pancreatic Beta Cells.- Primary Adipocytes as a Model for Insulin Sensitivity.- Assessing Islet Transplantation Outcome in Mice.- The Measurement of Insulin Secretion using Pancreas Perfusion in the Rodent.

Erscheinungsdatum	28.10.2019
Reihe/Serie	Methods in Molecular Biology ; 2076
Zusatzinfo	32 Illustrations, color; 18 Illustrations, black and white; X, 300 p. 50 illus., 32 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizinische Fachgebiete ► Innere Medizin ► Diabetologie
	Medizin / Pharmazie ► Studium
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
Schlagworte	Chemoinformatics • gene expression • Hyperinsulinemia • Insulin • whole exosome sequencing (WES)
ISBN-10	1-4939-9880-3 / 1493998803
ISBN-13	978-1-4939-9880-7 / 9781493998807
Zustand	Neuware