Post-Translational Modification of Proteins

Tools for Functional Proteomics

Buch | Hardcover

327 Seiten

2019 | 3rd ed. 2019
Humana Press Inc. (Verlag)
9781493990535 (ISBN)

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The majority of all proteins undergo co- and/or post-translational modifications, crucially altering physical and chemical properties, folding, conformation distribution, stability, activity, and, consequently, the function of the proteins. In Post-Translational Modifications of Proteins: Tools for Functional Proteomics, Third Edition this important area of research is brought up-to-date by the leading scientists in the field. This compilation of detailed protocols focuses on protein function, proteome research and characterization of pharmaceutical proteins, while following the successful format of the Methods in Molecular Biology series. Each chapter provides a brief introduction to the topic, step-by-step laboratory protocols, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting edge, Post-Translational Modifications of Proteins: Tools for Functional Proteomics,Third Edition serves as practical guide for researchers working in the field of protein structure-function relationships and the rapidly growing field of proteomics, as well as scientists in the pharmaceutical industries.

Web-Based Computational Tools for the Prediction and Analysis of Post-Translational Modifications of Proteins.- Detection of Post-Translational Modifications by Fluorescent Staining of Two-Dimensional Gels.- Analysis of Ser/Thr Linked Sugar Chains.- Enzymatic Release of Glycoprotein N-Glycans and Fluorescent Labeling.- Enzymatic Sequence Analysis of N-Glycans By Exoglycosidase Cleavage and Mass Spectrometry: Detection of Lewis X Structures.- Chromatographic Profiling of N-Glycans.- Activity Determination of Glycosyltransferases.- Characterization of Site-Specific N-Glycosylation.- Glycosylation of Type I Collagen.- Metabolic Labeling and Structural Analysis of Glycosylphosphatidylinositols from Parasitic Protozoa.- Identification of Protein Phosphorylation Sites by Advanced LC-ESI-MS/MS Methods.- Mass Spectrometry-Based Method for Detection and Identification of Free Thiol Groups in Proteins.- Mass Spectrometric Determination of Protein Ubiquitination.- Analysis of Sumoylation.- An Approach for the Identification of Proteins Modified by ISG15.- α-Amidated Peptides: Approaches for Analysis.- S-Acylation of Proteins.- Analysis of Methylation, Acetylation, and Other Modifications in Bacterial Ribosomal Proteins.- Lysine Hydroxylation and Cross-Linking of Collagen.

Erscheinungsdatum	18.07.2019
Reihe/Serie	Methods in Molecular Biology ; 1934
Zusatzinfo	31 Illustrations, color; 81 Illustrations, black and white; XI, 327 p. 112 illus., 31 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	Cell Culture • Phosphoproteomics • Protein function • protein-ligand binding • protein ubiquitination • Structure-function relationship
ISBN-13	9781493990535 / 9781493990535
Zustand	Neuware