Gene Transfer and Expression

A Laboratory Manual

Michael Kriegler (Autor)

Buch | Spiralbindung

256 Seiten

1993
W.H.Freeman & Co Ltd (Verlag)
978-0-7167-7004-6 (ISBN)

Infos

This book is a catalogue of viral and cis-acting elements and expression vectors for obtaining optimum expression of foreign genes introduced into mammalian cells. It describes techniques for propagating cells and cell lines, DNA transfer, drug selection and gene amplification, expression cloning, retro-virus mediated gene transfer.

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This book is a state-of-the-art catalogue of viral and cis-acting elements and the latest expression vectors for obtaining optimum expression of foreign genes introduced into mammalian cells. It describes in detail the essential techniques for propagating cells and cell lines, DNA transfer, drug selection and gene amplification, expression cloning, subtractive hybridizaton, retro-virus mediated gene transfer.

Inhalt

PART I: Gene Transfer
1: Eukaryotic Control Elements
2: Vectors
3: Processing of Proteins Encoded by Transferred Genes
PART II: Gene Transfer Methods
4: Cells and Cell Lines
5: DNA Transfer
6: Selection and Amplification
7: Expression Cloning
8: Subtractive Hybridization
9: Retrovirus-Mediated Gene Transfer
10: PCR-Based Expression
PART III: Assays for Gene Transfer and Expression
11: Assays for Gene Transfer
12: Assays for Gene Expression
Appendix: Suppliers

Produkt-Details

• Erscheint lt. Verlag: 30.9.1993
• Zusatzinfo: 29 illustrations
• Sprache: englisch
• Maße: 224 x 279 mm
• Gewicht: 632 g
• Themenwelt: Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Zoologie
• ISBN-10: 0-7167-7004-0 / 0716770040
• ISBN-13: 978-0-7167-7004-6 / 9780716770046
• Zustand: Neuware