Tumor Suppressor Genes

Volume 2: Regulation, Function, and Medicinal Applications

Buch | Hardcover

657 Seiten

2003 | 2003 ed.
Humana Press Inc. (Verlag)
978-0-89603-987-2 (ISBN)

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It has become clear that tumors result from excessive cell proliferation and a corresponding reduction in cell death caused by the successive accumulation of mutations in key regulatory target genes over time. During the 1980s, a number of oncogenes were characterized, whereas from the 1990s to the present, the emp- sis has shifted to tumor suppressor genes (TSGs). It has become clear that oncogenes and TSGs function in the same pathways, providing positive and negative growth regulatory activities. The signaling pathways controlled by these genes involve virtually every process in cell biology, including nuclear events, cell cycle, cell death, cytoskeletal, cell membrane, angiogenesis, and cell adhesion effects. Mu- tions in tumor suppressor genes have been identified in familial cancer syndromes, and the same genes in many cases have been found to be mutationally inactivated in sporadically occurring cancers. In their normal state, TSGs control cancer development and progression, as well as contribute to the sensitivity of cancers to a variety of therapeutics. Understanding the classes of TSGs, the biochemical pa- ways they function in, and how they are regulated provides an essential lesson in cancer biology. We cannot hope to advance our current knowledge and to develop new and more effective therapies without understanding the relevant pathways and how they influence the present approaches to therapy. Moreover, it is important to be able to access not only the powerful tools now available to discover these genes, but also their links to cell biology and growth control.

Understanding the Function and Regulation of Tumor Suppressor Genes.- Utilizing NMR to Study the Structure of Growth-Inhibitory Proteins.- Generation and Application of Phospho-specific Antibodies for p53 and pRB.- Stability and Ubiquitination of the Tumor Suppressor Protein p53.- Role of Tumor Suppressors in DNA Damage Response.- In Situ Hybridization in Cancer and Normal Tissue.- Genetic Strategies in Saccharomyces cerevisiae to Study Human Tumor Suppressor Genes.- Electrophoretic Mobility Shift Analysis of the DNA Binding of Tumor Suppressor Gene Products.- Analysis of Gene Promoter Regulation by Tumor Suppressor Genes.- DNA Footprinting.- Identification of DNA-Binding of Tumor Suppressor Genes by Chromatin Immunoprecipitation.- Co-immunoprecipitation of Tumor Suppressor Protein-Interacting Proteins.- Microarray Approaches for Analysis of Tumor Suppressor Gene Function.- Analysis of Tumor Suppressor Gene-Induced Senescence.- Yeast Two-Hybrid Screening as a Means of Deciphering Tumor Suppressor Pathways.- Somatic Cell Knockouts of Tumor Suppressor Genes.- Colony Growth Suppression by Tumor Suppressor Genes.- Flow Cytometric Analysis of Cell Cycle Control by Tumor Suppressor Genes.- Analysis of Cyclin-Dependent Kinase Activity.- Tumor Suppressor Gene-Inducible Cell Lines.- In Vitro Models of Early Neoplastic Transformation of Human Mammary Epithelial Cells.- Tumor Suppression Through Angiogenesis Inhibition.- Flow Cytometric Analysis of Tumor Suppressor Gene-Induced Apoptosis.- Functional Analysis of Tumor Suppressor Genes in Mice.- The Tissue Microenvironment as an Epigenetic Tumor Modifier.- Analyzing the Function of Tumor Suppressor Genes Using a Drosophila Model.- Assembling a Tumor Progression Model.- Conformation-Sensitive Gel Electrophoresis for Detecting BRCA1Mutations.- Medicinal Applications of Tumor Suppessors.- Conversion Technology and Cancer Predispositions.- Discovering Novel Anticancer Drugs.- Targets in Apoptosis Signaling.- Tumor Deprivation of Oxygen and Tumor Suppressor Gene Function.- Hormonal and Differentiation Agents in Cancer Growth Suppression.- Regulation of NF-?B by Oncoproteins and Tumor Suppressor Proteins.- Blocking Survivin to Kill Cancer Cells.- Targeting the Mitochondria to Enhance Tumor Suppression.- Novel Approaches to Screen for Anticancer Drugs Using Saccharomyces cerevisiae.- Tumor Suppressor Gene Therapy.- Therapeutic Strategies Using Inhibitors of Angiogenesis.- Isolation of p53 Inhibitors by Screening Chemical Libraries in Cell-Based Readout System.

Reihe/Serie	Methods in Molecular Biology ; 223
Zusatzinfo	XIX, 657 p.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie
	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
	Naturwissenschaften
ISBN-10	0-89603-987-0 / 0896039870
ISBN-13	978-0-89603-987-2 / 9780896039872
Zustand	Neuware