Rheumatoid Arthritis

Methods and Protocols

Buch | Hardcover

253 Seiten

2018 | 1st ed. 2018
Humana Press Inc. (Verlag)
978-1-4939-8801-3 (ISBN)

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This volume provides a summary of the standard laboratory protocols and methodology commonly used in basic and translational studies in the field of rheumatoid arthritis (RA) treatment. Chapters detail including basic RA models, evaluation of disease activity and immunological status, systemic drug delivery, and new research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Rheumatoid Arthritis: Methods and Protocols aims to be a basic manual for clinical researchers who are just getting started in the field of intervention study.

Collagen-induced Arthritis Models.- Human Xenograft Model.- Long-term Constant Subcutaneous Drug Administration.- Clinical Scoring of Disease Activity in Animal Models.- Histological Analysis of Arthritic Joints.- Preparation of Joint Extracts.- Production of Immunizing Antigen Proteoliposome Using Cell-free Protein Synthesis System.- Re-construction of Protein/Liposome Complex.- Production of Neutralizing Antibody.- Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreen.- Generation of Specific Aptamers.- Production of Lentiviral Particles.- RNA Interference Ex Vivo.- Lentiviral-mediated Systemic RNA Interference in vivo.- Mesenchymal Stem Cell Engineering.- Screening of Ca2+ Influx in Lymphocytes.- Single-cell Ca2+ Imaging.- Electrophysiological Methods to Measure Ca2+ Current.- The Functional Assessment of T cells.- Release of Antibodies and Cytokines from B cells.- Evaluation of Autoreactive Responses.- Bone-resorption Activity in Mature Osteoclasts.- Animal Models of Vasculitis.- Design an Intervention Study.- Assessment of Disease Activity, Structural Damage, and Function in Rheumatoid Arthritis.

Erscheinungsdatum	07.10.2018
Reihe/Serie	Methods in Molecular Biology ; 1868
Zusatzinfo	34 Illustrations, color; 25 Illustrations, black and white; IX, 253 p. 59 illus., 34 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizinische Fachgebiete ► Innere Medizin ► Rheumatologie
	Medizin / Pharmazie ► Studium
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
Schlagworte	Aptamers • Autoimmune inflammation • drug development • Functional antibodies • Viral-mediated gene therapy
ISBN-10	1-4939-8801-8 / 1493988018
ISBN-13	978-1-4939-8801-3 / 9781493988013
Zustand	Neuware