Experimental Neuroanatomy

A Practical Approach

Buch | Softcover

296 Seiten

1992
Oxford University Press (Verlag)
978-0-19-963325-8 (ISBN)

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A dramatic increase in the number of practical techniques available to the experimental neuroanatomist have led to major advances in the knowledge and understanding of the nervous system. This book is a laboratory manual for experimental neuroscientists, with an emphasis on ultrastructural analysis. It contains detailed protocols for the preparation of specimens, perfusion-fixation, and the preparation of sections for light and electron microscopy. It covers neuroanatomical techniques including procedures for tract-tracing and chemical characterization of neurones and their processes. These procedures, either alone or in combination, are being used to elucidate complex neuronal interactions. This text should be useful to neuroanatomists and other neuroscientists who wish to apply these experimental techniques.

J.P. Bolam: Preparation of central nervous system tissue for light and electron microscopy; I.J. Llewellyn-Smith, P. Pilowsky, & J.B. Minson: Retrograde tracers for light and electron microscopy; Y. Smith: Anterograde tracing with PHA-L and biocytin at the electron microscopic level; P.L. Strick & J.P. Card: Transneuronal mapping of neural circuits with alpha herpesviruses; S. Totterdell, C.A. Ingham, & J.P. Bolam: Immunocytochemistry I: pre-embedding
staining; C.A. Ingham: Immunocytochemistry II: post-embedding staining; S.R. Vincent: Histochemistry of endogenous enzymes; C.R. Gerfen, W.S. Young, III, & P. Emson: In situ hybridization histochemistry with oligonucleotides for localization of messenger RNA in neurones; E.H. Buhl: Intracellular Lucifer Yellow
injection in fixed brain slices; D.J. Maxwell: Intracellular staining in vivo; Y. Smith, & J.P. Bolam: Combined approaches to experimental; neuroanatomy: combined tracing and immunocytochemical techniques for the study of neuronal microcircuits; Appendix; Index.

Erscheint lt. Verlag	17.12.1992
Reihe/Serie	Practical Approach Series ; 114
Zusatzinfo	41 halftones, 5 line drawings, tables
Verlagsort	Oxford
Sprache	englisch
Maße	159 x 236 mm
Gewicht	456 g
Themenwelt	Studium ► 1. Studienabschnitt (Vorklinik) ► Anatomie / Neuroanatomie
Themenwelt	Naturwissenschaften
ISBN-10	0-19-963325-8 / 0199633258
ISBN-13	978-0-19-963325-8 / 9780199633258
Zustand	Neuware