Experimental Cell and Molecular Biology

John S. Choinski (Autor)

Loseblattwerk

192 Seiten

1992 | 2nd Revised edition
Brown (William C.) Co ,U.S. (Verlag)
9780697158925 (ISBN)

Infos

This textbook provides a series of laboratory based exercises in all areas of cellular and molecular biology for students at introductory level. Four laboratories have been added to this second edition, introducing students to the latest techniques in cell and molecular exercises.

Inhalt

Introduction to data gathering and analysis; liposome formation as a model for cell evolution; some observations and exercises concerning the use and care of light microscopes; light microscope observations of cell structure using specific staining techniques; growth kinetics in yeast cell culture; pH and buffers; spectrophotometry; introduction to the quantitative assay of enzymatic activity: triose phosphate isomerase; the kinetic characterisation of alcohol dehydrogenase; rat liver fractionation by differential centrifugation; analysis of rat liver mitochondrial succinate dehydrogenase; isolation of castor bean endosperm organelles using density gradient centrifugation; assay of lecithin synthesis by cholinephosphotransferase; determination of molecular weight using SDS-PAGE: separation of proteins using gel filtration chromatography; investigation of transcription in isolated rat liver nuclei; light-driven proton pumping in spinach chloroplasts; translation in pea chloroplasts; restriction endonuclease analysis of nuclear DNA; immunocytochemical analysis of the cytoskeleton of cultured animal cells.

Produkt-Details

• Erscheint lt. Verlag: 1.10.1996
• Zusatzinfo: Illustrations
• Verlagsort: Dubuque, IA
• Sprache: englisch
• Maße: 224 x 280 mm
• Gewicht: 550 g
• Themenwelt: Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Zellbiologie
• ISBN-13: 9780697158925 / 9780697158925
• Zustand: Neuware