Photosynthesis

Methods and Protocols

Buch | Hardcover

355 Seiten

2018 | 2018 ed.
Humana Press Inc. (Verlag)
9781493977857 (ISBN)

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This user-friendly book provides a range of classical and modern techniques for the study of photosynthesis in a manner accessible to a broad spectrum of researchers. Broken into four sections, it explores the measurement of physiological photosynthetic parameters, quantifying photosynthetic enzyme abundance and catalytic activity, visualizing cellular and sub-cellular phenotypes, and photosynthesis-inspired energy generation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Photosynthesis: Methods and Protocols aims to aid scientists working toward exciting developments in the understanding and application of photosynthesis.

Survey of Tools for Measuring In Vivo Photosynthesis.- Photosynthetic Gas Exchange in Land Plants at the Leaf Level.- Design and Use of a Digitally-Controlled Device for Accurate, Multiplexed Gas Exchange Measurements of the Complete Foliar Parts of Plants.- Measuring Canopy Gas Exchange Using CAnopy Photosynthesis and Transpiration Systems (CAPTS).- Light-Response Curves in Land Plants.- Chlorophyll Fluorescence on the Fast Timescale.- Sub-Saturating Multiphase Flash Irradiances to Estimate Maximum Fluorescence Yield.- Chlorophyll Fluorescence Imaging.- Measurement of O2 Uptake and Evolution in Leaves In Vivo Using Stable Isotopes and Membrane Inlet Mass Spectrometry.- Using Carbon Stable Isotopes to Study C3 and C4 Photosynthesis: Models and Calculations.- Liquid-Phase Measurements of Photosynthetic Oxygen Evolution.- Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting.- Extraction of Rubisco to Determine Catalytic Constants.- Spectrophotometric Determination of Rubisco Activity and Activation State in Leaf Extracts.- Creating Leaf Cell Suspensions for Characterization of Mesophyll and Bundle Sheath Cellular Features.- Determining the Subcellular Localization of Fluorescently-Tagged Proteins Using Protoplasts Extracted from Transiently-Transformed Nicotiana benthamiana Leaves.- 3D Clearing and Molecular Labeling in Plant Tissues.- Evaluation of Lipids for the Study of Photosynthetic Membranes.- “Click” Methodology for the Functionalization of Water Oxidation Catalyst Iridium Oxide Nanoparticles with Hydrophobic Dyes for Artificial Photosynthetic Constructs.- Biophotovoltaics: Design and Study of Bio-Electrochemical Systems for Biotechnological Applications and Metabolic Investigation.

Erscheinungsdatum	25.06.2018
Reihe/Serie	Methods in Molecular Biology ; 1770
Zusatzinfo	47 Illustrations, color; 23 Illustrations, black and white; XI, 355 p. 70 illus., 47 illus. in color. With online files/update.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Botanik
Schlagworte	Chlorophyll • Enzyme abundance • Oxygen evolution • Photosynthesis-inspired energy generation • Photosynthetic parameters
ISBN-13	9781493977857 / 9781493977857
Zustand	Neuware