Plant Signal Transduction

Methods and Protocols

Jose R. Botella, Miguel A. Botella (Herausgeber)

Buch | Softcover

241 Seiten

2016 | Softcover reprint of the original 2nd ed. 2016
Humana Press Inc. (Verlag)
978-1-4939-4959-5 (ISBN)

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This fully updated volume reflects the spectacular advances in our knowledge of signal transduction pathways with a selection of ‘classic’ as well as newly developed approaches. These detailed approaches expand into the fields of molecular biology, biochemistry, physiology, cell biology, genetics, and genomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and up-to-date, Plant Signal Transduction: Methods and Protocols, Second Edition serves as an ideal guide for researchers exploring the vast array of signals produced by plants to ensure their survival.

Suppressor Screens in Arabidopsis.- Quantitative Reverse Transcription-qPCR-Based Gene Expression Analysis in Plants.- DNA-Binding Factor Target Identification by Chromatin Immunoprecipitation (ChIP) in Plants.- Identification of Genes Responsible for Natural Variation in Volatile Content Using Next Generation Sequencing Technology.- Micro-Tom Tomato as an Alternative Plant Model System: Mutant Collection and Efficient Transformation.- Culture of the Tomato Micro-Tom Cultivar in Greenhouse.- Virus-Induced Gene Silencing as a Tool to Study Tomato Fruit Biochemistry.- A Simplified and Rapid Method for the Isolation and Transfection of Arabidopsis Leaf Mesophyll Protoplasts for Large-Scale Applications.- Preparation of Epidermal Peels and Guard Cell Protoplasts for Cellular, Electrophysiological, and -Omics Assays of Guard Cell Function.- Application of Variable Angle Total Internal Reflection Fluorescence Microscopy to Investigate Protein Dynamics in Intact Plant Cells.- Immunoprecipitation of Plasma Membrane Receptor-Like Kinases for Identification of Phosphorylation Sites and Associated Proteins.- Yeast Three-Hybrid System for the Detection of Protein-Protein Interactions.- Cautions in Measuring In Vivo Interactions Using FRET and BiFC in Nicotiana benthamiana.- Analysis of Protein-Lipid Interactions Using Purified C2 Domains.- Assessing Kinase Activity in Plants with In-Gel Kinase Assays.- Analyses of Plant UDP-Dependent Glycosyltransferases to Identify Their Volatile Substrates Using Recombinant Proteins.- Competitive Index: Mixed Infection-Based Virulence Assays for Genetic Analysis in Pseudomonas syringae-Plant Interactions.- Simplified Assays for Evaluation of Resistance to Alternaria brassicicola and Turnip Mosaic Virus.- Simultaneous Determination of Plant Hormones by GC-TOF-MS.

Erscheinungsdatum	19.08.2017
Reihe/Serie	Methods in Molecular Biology ; 1363
Zusatzinfo	XV, 241 p.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Botanik
Schlagworte	Arabidopsis • Crop improvement • Mutant gene production • Plant receptors • signaling pathway • Signal transduction pathways • Tomato models
ISBN-10	1-4939-4959-4 / 1493949594
ISBN-13	978-1-4939-4959-5 / 9781493949595
Zustand	Neuware