Controlled Genetic Manipulations

Buch | Softcover

227 Seiten

2016 | Softcover reprint of the original 1st ed. 2012
Humana Press Inc. (Verlag)
9781493961962 (ISBN)

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The current demand for the development of techniques for controlled genetic manipulations is driven by the anatomical and physiological complexity of the brain and by the need for experimental models that can address this complexity through selective manipulation of defined components of the system: specific neuronal populations or selected synapses. In Controlled Genetic Manipulations, expert researchers present detailed chapters to supply basic technical information about controlled genetic manipulations and to provide examples of creative implementation of this methodology when addressing a unique biological problem. Some chapters of the book describe the most recent developments in the basic methodology, which includes use of Cre-recombinase, methods for delivery of genetic material into the brain and the use of optogenetics, whereas other chapters focus on applying these techniques to addressing particular biological questions like structural and functional mapping of neuronal circuits, analysis of specific synaptic connections, modeling or gene therapy of neurological disorders. As part of the Neuromethods series, chapters include key implementation advice that is crucial for getting optimal results.

Authoritative and practical, Controlled Genetic Manipulations serves as a valuable guide to a variety of techniques, provided by many of the pioneers of the approaches.

Regulation of Cre Recombinase: Use of Ligand-Regulated and Dimerizable Cre for Transgenesis.- Genetic Manipulations Using Cre and Mutant loxP Sites.- Using Recombinant Adeno-Associated Viral Vectors for Gene Expression in the Brain.- Using Lentiviral Vectors as Delivery Vehicles for Gene Therapy.- Targeting Neurological Disease with siRNA.- In Utero Electroporation for Cellular Transgenesis in the Developing Mammalian Forebrain.- Single-Cell Electroporation of siRNA in Primary Neuronal Cultures.- Development and Application of Membrane-Tethered Toxins for Genetic Analyses of Neuronal Circuits.- Genetic Methods for Anatomical Analysis of Neuronal Circuits.- Functional Circuitry Analysis in Rodents Using Neurotoxins/Immunotoxins.- Analysis of Neuronal Circuits with Optogenetics.

Erscheinungsdatum	19.08.2017
Reihe/Serie	Neuromethods ; 65
Zusatzinfo	27 Illustrations, color; 21 Illustrations, black and white; XII, 227 p. 48 illus., 27 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
	Naturwissenschaften ► Biologie ► Humanbiologie
	Naturwissenschaften ► Biologie ► Zoologie
ISBN-13	9781493961962 / 9781493961962
Zustand	Neuware