Techniques in Molecular Biology

Buch | Softcover

335 Seiten

1983 | Softcover reprint of the original 1st ed. 1983
Kluwer Academic Publishers (Verlag)
978-0-7099-2755-6 (ISBN)

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The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.

1. High Performance Liquid Chromatography of Proteins and Nucleic Acids.- 2. Analytical Electrophoretic Techniques in Protein Chemistry.- 3. Protein Blotting.- 4. The Production and Isolation of Peptides for Sequence Analysis.- 5. Protein and Peptide Sequence Determination.- 6. The Extraction and Fractionation of RNA.- 7. Protein Synthesis In Vitro.- 8. Labelling DNA In Vitro — Nick Translation.- 9. Synthesis of cDNA for Molecular Cloning.- 10. Plasmid Isolation.- 11. The Use of Restriction Endonucleases and T4 DNA Ligase.- 12. Molecular Cloning of cDNA: Bacterial Transformation and Screening of Transformants.- 13. The Use of Minicells and Maxicells to Detect the Expression of Cloned Genes.- 14. Gel Electrophoresis of DNA.- 15. Detection of Specific DNA Sequences — The Southern Blot.- 16. The Determination of DNA Sequences.- 17. The Use of Cosmids as Cloning Vehicles.- Notes on Contributors.

Zusatzinfo	335 p.
Verlagsort	Dordrecht
Sprache	englisch
Maße	155 x 235 mm
Themenwelt	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
Themenwelt	Naturwissenschaften ► Biologie ► Zoologie
ISBN-10	0-7099-2755-X / 070992755X
ISBN-13	978-0-7099-2755-6 / 9780709927556
Zustand	Neuware