Time-Resolved Mass Spectrometry (eBook)

From Concept to Applications

Pawel L. Urban, Yu-Chie Chen, Yi-Sheng Wang (Autoren)

eBook Download: EPUB

2016
John Wiley & Sons (Verlag)
978-1-118-88735-6 (ISBN)

Infos

Time is an important factor in physical and natural sciences. It characterizes the progress of chemical and biochemical processes. Mass spectrometry provides the means to study molecular structures by detecting gas-phase ions with the unique mass-to-charge ratios. Time-resolved mass spectrometry (TRMS) allows one to differentiate between chemical states that can be observed sequentially at different time points. Real-time mass spectrometric monitoring enables recording data continuously with a specified temporal resolution. The TRMS approaches - introduced during the past few decades - have shown temporal resolutions ranging from hours down to microseconds and beyond.
This text covers the key aspects of TRMS. It introduces ion sources, mass analyzers, and interfaces utilized in time-resolved measurements; discusses the influence of data acquisition and treatment; finally, it reviews most prominent applications of TRMS - in the studies of reaction kinetics and mechanism, physicochemical phenomena, protein structure dynamics, biocatalysis, and metabolic profiling.
It will assist science and engineering students to gain a basic understanding of the TRMS concept, and to recognize its usefulness. In addition, it may benefit scientists who conduct molecular studies in the areas of chemistry, physics and biology.

Autor

Pawel Urban is an Assistant Professor in the Department of Applied Chemistry at National Chiao Tung University, Taiwan. He studied his MISMaP MSc, in biology at the University of Warsaw, Poland between 1999 & 2002. He then studied at the University of York UK, between 2004 & 2007 where he gained his PhD, in Chemistry. He began working as a postdoctoral Assistant in the Faculty of Biology, University of Warsaw, before moving to Swiss Federal Institute of Technology (ETH), Zurich and 2 years later to National Chiao Tung University, where in both institutes he?worked as a PDRA, before beginning his current role of Assistant Professor. Yu-Chie Chen is a Professor of Chemistry in the Department of Applied Chemistry, National Chiao Tung University, Taiwan. She obtained her Bachelor degree at Kung University, Taiwan, her Masters at Sun Yat-sen, Taiwan and her P.h.D at Montana State University, USA. Before working at National Chiao Tung University, she held positions at Swiss Federal Institute of Technology Zurich, Tzu Chi Medical and Tzu Chi University. Yi-Sheng Wang is an Associate Research Fellow at the Genomics Research Center, Academia Sinica, Taiwan. He acheived his Chemistry Ph.D. at the National Taiwan University, in 2001. Between 2001 & 2005, he began working as a?Postdoctoral Fellow, at the Institute of Atomic and Molecular Sciences (IAMS), Academia Sinica, (including a Postdoctoral Fellow at?National High Magnetic Field Laboratory (NHMFL), Florida State University, U.S.A. between 2002-2003). In 2005, he moved to the Genomics Research Center, Academia Sinica, Taiwan, as a Assistant Research?Fellow, until 2011, when he was promoted to?his current position of Associate Research Fellow.

Textauszug

List of Acronyms

2D	two-dimensional

3D	three-dimensional

ABS	acrylonitrile butadiene styrene

AC	alternating current

ADP	adenosine diphosphate

AMS	aerosol mass spectrometry

APCI	atmospheric pressure chemical ionization

API	atmospheric pressure ionization

APPI	atmospheric pressure photoionization

ASAP	atmospheric solids analysis probe

ATP	adenosine triphosphate

BIRD	blackbody infrared radiative dissociation

CAD	computer-aided design

C-API

contactless atmospheric pressure ionization

CCS	collision cross-section

CE	capillary electrophoresis

CEC	capillary electrochromatography

CFA	continuous flow analysis

CGE	capillary gel electrophoresis

CI	chemical ionization

CID	collision-induced dissociation

cITP	capillary isotachophoresis

CSI	cold spray ionization

CTI	charge-transfer ionization

CZE	capillary zone electrophoresis

DAPCI

desorption atmospheric pressure chemical ionization

DART	direct analysis in real time

DBDI	dielectric barrier discharge ionization

DC	direct current

DESI	desorption electrospray ionization

DHB	2,5-dihydroxybenzoic acid

DIOS	desorption/ionization on silicon

DMF	digital microfluidics

EASI	easy ambient sonic-spray ionization

ECD	electron capture dissociation

EESI	extractive electrospray ionization

EI	electron ionization

EIC	extracted-ion current

EKC	electrokinetic chromatography

ELDI	electrospray-assisted laser desorption/ionization

ELISA

electrostatic ion storage ring, Aarhus

EM	electron multiplier

EOF	electroosmotic flow

ESI	electrospray ionization

ESSI	electrosonic spray ionization

ETD	electron transfer dissociation

EWOD	electrowetting-on-dielectric

FAB	fast atom bombardment

FAPA	flowing atmospheric-pressure afterglow

FIA	flow-injection analysis

FID	flame ionization detection

FPOP	fast photochemical oxidation of proteins

FT	Fourier transform

FWHM	full width at half maximum

GC	gas chromatography

HDX	hydrogen/deuterium exchange

HETP	height equivalent to a theoretical plate

HILIC

hydrophilic interaction chromatography

HPLC	high-performance liquid chromatography

HV	high voltage

ICAT	isotope-coded affinity tag

ICP	inductively coupled plasma

ICR	ion cyclotron resonance

IM	ion mobility

IMAC	immobilized metal ion affinity chromatography

IMS	ion-mobility spectrometry

IR

infrared

IRMPD

infrared multiphoton dissociation

ISD	in-source decay

IT

ion trap

LAESI

laser ablation electrospray ionization

LC	liquid chromatography

LDI	laser desorption/ionization

LIT	linear ion trap

LMJ	liquid microjunction

LOD	limit of detection

LTP	low-temperature plasma

MALDI

matrix-assisted laser desorption/ionization

MAMS	micro-arrays for mass spectrometry

MCP	microchannel plate

MEKC	micellar electrokinetic chromatography

MIMS	membrane inlet mass spectrometry

MPI	multiphoton ionization

MRM	multiple reaction monitoring

MS	mass spectrometry

MudPIT

multidimensional protein identification technology

nanoDESI

nanospray desorption electrospray ionization

nanoESI

nanospray electrospray ionization

NBDPZ

4-nitro-7-piperazino-2,1,3-benzoxadiazole

NIMS	nanostructure-initiator mass spectrometry

NMR	nuclear magnetic resonance

NP-LC

normal-phase liquid chromatography

OIT	orbital ion trap

PA	proton affinity

PAT	process analytical technology

PDMS	polydimethylsiloxane

PE	potential energy

PEG	polyethylene glycol

PESI	probe electrospray ionization

PET	polyethylene terephthalate

PFTBA

perfluorotri-n-butylamine

PICT	photoionization charge transfer

PI-ESI

polarization induced electrospray ionization

PMMA	poly(methyl methacrylate)

PSD	post-source decay

PTR	proton-transfer reaction

Q	quadrupole

QMF	quadrupole mass filter

QqQ	triple quadrupole

RF	radio frequency

RMS	root-mean-square

RP	reversed phase

RP-LC

reversed-phase liquid chromatography

S

sector

SALDI

surface-assisted laser desorption/ionization

SAT	spectrum acquisition time

SDS	sodium dodecyl sulfate

SEC	size-exclusion chromatography

SID	surface-induced dissociation

SIFT	selected ion flow tube

SILAC

stable isotope labeling by amino acids in cell culture

SIM	selected ion monitoring

SIMS	secondary ion mass spectrometry

SPE	solid-phase extraction

SPME	solid-phase microextraction

S/N	signal-to-noise

SPI	single photon ionization

SRM	selected reaction monitoring

SSP	surface sampling

TE	translational...

Produkt-Details

• Erscheint lt. Verlag: 19.1.2016
• Sprache: englisch
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Themenwelt: Naturwissenschaften ► Chemie ► Analytische Chemie
• Themenwelt: Technik
• Schlagworte: Analytical Chemistry • Biochemie • biochemistry • Biomolecules (DNA, RNA, Peptides, etc.) • Biomolekül • Biomoleküle (DNA, RNA, Peptide) • Biomolekül • Biomoleküle (DNA, RNA, Peptide) • Biowissenschaften • Chemical Analysis • Chemie • Chemistry • Instrumentation • Life Sciences • Massenspektrometrie • Mass Spectrometry • molecular dynamics • Reaction kinetics • Real-time Monitoring • Temporal Resolution • Time-resolved measurements
• ISBN-10: 1-118-88735-2 / 1118887352
• ISBN-13: 978-1-118-88735-6 / 9781118887356