Plant Microtechniques and Protocols (eBook)

Edward Chee Tak Yeung, Claudio Stasolla, Michael John Sumner, Bing Quan Huang (Herausgeber)

eBook Download: PDF

2015
576 Seiten
Springer International Publishing (Verlag)
978-3-319-19944-3 (ISBN)

Infos

A proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one’s own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e.g., Jensen (1964); O’Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e.g., archeology, wood science, etc.]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.​

Autor

Edward C. YeungDepartment of Biological Sciences, University of Calgary, Calgary, Alberta, CanadaClaudio StasollaDepartment of Plant Science, University of Manitoba, Winnipeg, Manitoba, CanadaMichael J. SumnerDepartment of Biological Sciences, University of Manitoba, Winnipeg, Manitoba, CanadaBing Q. HuangMayo Clinic, Rochester, Minnesota, USA

Inhalt

Contents PrefaceContributors Part I   Fixation, Processing, Embedding and Staining of Botanical Specimens  1.      A Guide to the Study of Plant Structure with Emphasis on Living SpecimensEdward C. Yeung     2.      Chemical and Physical Fixation of Cells and Tissues: an OverviewBing Q. Huang and Edward C. Yeung   3.      Paraffin and Polyester WaxesClaudio Stasolla and Edward C. Yeung  4.      The Glycol Methacrylate Embedding Resins – Technovit 7100 and 8100Edward C. Yeung and Colin K. W. Chan 5.      Epoxy Resins for Light and Transmission Electron MicroscopyMichael J. Sumner 6.      LR White Acrylic ResinEdward C. Yeung and Bing Q. Huang 7.      High Pressure Freezing and Freeze Substitution of In vivo and In vitro Cultured Plant SamplesJose M. Seguí-Simarro 8.      Three-Dimensional Imaging for Electron Microscopy of Plastic-embedded Plant SpecimensJose M. Seguí-Simarro 9.      Fluorescent Staining of Living Plant Cells Sarah Schoor, Shiu-Cheung Lung, Dustin Sigurdson, and Simon D. X. Chuong 10.  Improved Methods for Clearing and Staining of Plant SamplesAlexander Lux, Marek Vaculík, and Ján Kováč Part II  Microtechniques related to Cell and Molecular biology studies 11.  Whole Mount Immunofluorescence Staining of Plant Cells and Tissues Masaki Shimamura                                                  12.  Protoplast Isolation and StainingShiu-Cheung Lung, Sarah Schoor, Dustin Sigurdson, Makoto Yanagisawa, Kelly Yeung, Mi Qi Liu and Simon D. X. Chuong 13.  Guiding Principles for Live Cell Imaging of Plants using Confocal MicroscopySubramanian Sankaranarayanan and Marcus A. Samuel                                                                                                          14.  Immunogold Labeling for Electron Microscopy: Strategy and Problem SolvingFengli Guo and Bing Q. Huang 15.  Abscisic Acid ImmunostainingYung-I Lee and Mei-Chu Chung 16.  Plant Chromosome Preparations and Staining for Light Microscopic StudiesSubhash C. Hiremath and C.C. Chinnappa  17.  Chromosome techniques and FISHMei-Chu Chung 18.  Detection of S-phase of Cell Division Cycle in Plant Cells and Tissues by using 5-ethynyl-2’-deoxyuridine (EdU)Soujanya Kuntam and Ferhan Ayaydin 19.  Staining Methods for Programmed Cell DeathOwen S. D. Wally and Claudio Stasolla 20.  Laser Microdissection of Plant Tissues Jenna L. Millar, Michael G. Becker, and Mark F. Belmonte                                                                                                          21.  RNA In situ HybridizationShalini Mukherjee, Claudio Stasolla, and Belay Ayele Part III   Preparative Methods for the Study of Wood Anatomy 22.  Microscopic Examination of Wood: Sample Preparation and Techniques for Light MicroscopyJacques C. Tardif and France Conciatori                                                                                                              23.  Collecting and Processing Wood Microcores for Monitoring XylogenesisAnnie Deslauriers, Sergio Rossi, and Eryuan Liang 24.  Three-dimensional Imaging of Cambium and Secondary Xylem Cells by Confocal Laser Scanning MicroscopySatoshi Nakaba, Peter Kitin, Yusuke Yamagishi, Shahanara Begum, Kayo Kudo, Widyanto Dwi Nugroho, and Ryo Funada Part IV   Botanical Techniques and Protocols for Archeology and Herbarium Collection 25.  Collection of Plant Remains from Archaeological ContextsAlessandra Celant, Donatella Magri, and Francesca Romana Stasolla 26.  Archaeological Wood Preparation Alessandra Celant and Gemma Coccolini 27.  Archaeopalynological Preparation Techniques Donatella Magri and Federico Di Rita 28.  Phytoliths: Preparation and Archaeological Extraction Brian Kooyman 29.  Starch Granules: Preparation and Archaeological Extraction Brian Kooyman        30.  Plant Collection, Identification, and Herbarium ProceduresBonnie Smith and C. C. Chinnappa  Index   

Produkt-Details

• Erscheint lt. Verlag: 19.9.2015
• Zusatzinfo: XIV, 576 p. 88 illus., 68 illus. in color.
• Verlagsort: Cham
• Sprache: englisch
• Themenwelt: Naturwissenschaften ► Biologie ► Botanik
• Themenwelt: Technik
• Schlagworte: Archeology • cellular and molecular biology studies • plant microtechniques • staining procedures • systematic botany • wood preparative methods
• ISBN-10: 3-319-19944-7 / 3319199447
• ISBN-13: 978-3-319-19944-3 / 9783319199443