The BAM Complex
Humana Press Inc. (Verlag)
9781493928705 (ISBN)
Authoritative and cutting-edge, The BAM Complex: Methods and Protocols will serve as an invaluable reference for those interested in studying the BAM complex.
The β-barrel Assembly Machinery Comple.- Yeast Mitochondria as a Model System to Study the Biogenesis of Bacterial β-barrel Proteins.- Experimental Methods for Studying the BAM Complex in Neisseria meningitides.- Heat Modifiability of Outer Membrane Proteins from Gram-Negative Bacteria.- The Role of a Destabilized Membrane for OMP Insertion.- Treponema pallidum in Gel Microdroplets: A Method for Topological Analysis of BamA (TP0326) and Localization of Rare Outer Membrane Proteins.- Analyzing the Role of Periplasmic Folding Factors in the Biogenesis of OMPs and Members of the Type V Secretion System.- An In Vitro Assay for Substrate Translocation by FhaC in Liposomes.- Measuring Cell-cell Binding Using Flow-Cytometry.- Methods to Characterize Folding and Function of BamA Crosslink Mutants.- Small Angle X-ray Scattering (SAXS) Characterization of the POTRA Domains of BamA.- Assessing the Outer Membrane Insertion and Folding of Multimeric Transmembrane β-barrel Proteins.- The Expression, Purification, and Structure Determination of BamA from E. coli.- Expression and Purification of the Individual Bam Components BamB-E.- Structure Determination of the BAM Complex Accessory Lipoproteins BamB-E.- An In Vitro Assay for Outer Membrane Protein Assembly by the BAM complex.- Identification of BamC on the Surface of E. coli.- Construction and Characterization of an E. coli bamD Depletion Strain.- Expression, Purification and Screening of BamE, a Component of the BAM Complex, for Structural Characterization.- Purification and Bicelle Crystallization for Structure Determination of the E. coli Outer Membrane Protein TamA.- Strategies for the Analysis of Bam Recognition Motifs in Outer Membrane Proteins.- Summary and Future Directions.
| Erscheint lt. Verlag | 2.10.2015 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1329 |
| Zusatzinfo | 16 Illustrations, color; 27 Illustrations, black and white; XI, 283 p. 43 illus., 16 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| Schlagworte | BAM complex • crystallization and structure determination • functional complex • Gram-negative bacteria • individual Bam components • mitochondria |
| ISBN-13 | 9781493928705 / 9781493928705 |
| Zustand | Neuware |
| Informationen gemäß Produktsicherheitsverordnung (GPSR) | |
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