Molecular Genetic Modification of Eucaryotes (eBook)

Charles E. Green, Ronald L. Phillips, Irwin Rubenstein (Herausgeber)

eBook Download: PDF

2013 | 1. Auflage
186 Seiten
Elsevier Science (Verlag)
978-1-4832-7235-1 (ISBN)

Infos

Molecular Genetic Modification of Eucaryotes reviews the current state of knowledge and techniques potentially useful for the molecular genetic modification of eucaryotes. The book interfaces molecular genetics, plant cell and tissue culture, and plant improvement. Topics include cell and tissue culture, protoplasts, somatic cell fusion, cellular mutagenesis, regeneration of new plant types, and the applicability of these techniques to plant improvements. Viruses and viral integration; integration and expression of foreign genetic material in human cells, Drosophila, plant cells, and legumes; biophysical studies of DNA uptake by plants; and genetic engineering for plant protection against diseases are also discussed in detail. The book will be of interest to geneticists, cell biologists, plant breeders, plant physiologists, plant pathologists, and biochemists.

Inhalt

Front Cover 1
Molecular Genetic Modification of Eucaryotes 4
Copyright Page 5
Table of Contents 8
Contributors 10
Preface 12
SECTION I: CELLULAR ASPECTS 16
Chapter 1. Protoplasts and Somatic Cell Hybridization in Plants 16
PROTOPLAST ISOLATION AND CULTURE 16
PLANT REGENERATION 24
CONCLUSIONS 24
REFERENCES 25
Chapter 2. Opportunities and Obstacles in the Culture of Cereal Protoplasts and Calluses 28
PREVIOUS WORK ON CULTURE OF MONOCOTYLEDONOUS CALLUSES, CELLS, AND PROTOPLASTS 29
CULTURE OF OAT PROTOPLASTS 31
CULTURE OF OAT CALLUS TISSUES 35
MACROMOLECULAR SYNTHESIS IN OAT PROTOPLASTS 38
RESULTS 39
RESPONSE OF OAT PROTOPLASTS TO THE PHYTOTOXIN VICTORIN 44
DISCUSSION AND CONCLUSIONS 52
ACKNOWLEDGEMENTS 52
REFERENCES 52
Chapter 3. Novel Cellular Associations 58
CHIMERAL PLANTS 58
PLANT-BACTERIAL ASSOCIATIONS 63
ACKNOWLEDGEMENTS 70
REFERENCES 70
Chapter 4. Isolation of Biochemical Mutants of Cultured Plant Cells 72
REFERENCES 79
Chapter 5. The Applicability of Plant Cell and Tissue Culture Techniques to Plant Improvement 82
REFERENCES 90
SECTION II: GENETIC ASPECTS 92
Chapter 6. Transducing Viruses and Viral Integration: Techniques for Genetic Modification 92
THE INTEGRATION AND EXCISION OF BACTERIOPHAGE LAMBDA 92
THE FORMATION OF TRANSDUCING PHAGE 93
EXTENDING THE RANGE OF LAMBDA TRANSDUCTION 93
REFERENCES 97
Chapter 7. Interactions of Bacterial Viruses and Bacterial Genes With Animal Systems 98
ACKNOWLEDGEMENT 109
REFERENCES 109
APPENDIX A: Phage Gene Directed Enzyme Activity in Genetically Defective Human Cells 111
REFERENCES 113
APPENDIX B: Phage - Mediated Transgenosis in Human Galactosemic Fibroblasts 113
REFERENCES 115
Chapter 8. Gene Transfer in Drosophila melanogaster 116
EXPERIMENTAL STRATEGIES 116
DROSOPHILA AS AN EXPERIMENTAL SYSTEM 117
SUMMARY OF EXPERIMENTAL RESULTS 120
WORK FROM OTHER LABORATORIES 140
INTERPRETATIONS 141
FUTURE TASKS AND IMPLICATIONS 143
ACKNOWLEDGEMENTS 144
REFERENCES 144
Chapter 9. Phage-Mediated Transgenosis in Plant Cells 148
REFERENCES 149
Chapter 10. Physical and Biological Studies of DNA Uptake by Plants 152
CsCl DENSITY GRADIENT ANALYSIS 153
DNA-DNA REASSOCIATION ANALYSIS 156
BACTERIAL TRANSFORMATION ASSAYS 158
DISCUSSION 158
SUMMARY 161
ACKNOWLEDGEMENTS 161
REFERENCES 161
Chapter 11. Molecular Genetic Modification of Legumes 164
BIOCHEMICAL AND GENETIC ASPECTS OF SYMBIOTIC DINITROGEN FIXATION IN LEGUMES 165
MOLECULAR MODIFICATION OF DINITROGEN FIXATION IN LEGUMES 168
EXPERIMENTAL RESULTS 171
AKNOWLEDGEMENTS 172
REFERENCES 172
Chapter 12. New Genetic Approaches to Plant Protection Against Diseases 174
PATHOGEN MANIPULATION 175
CULTURED HOST CELLS 177
REFERENCES 180
Index 182

Produkt-Details

• Erscheint lt. Verlag: 3.9.2013
• Sprache: englisch
• Themenwelt: Naturwissenschaften ► Biologie ► Evolution
• Themenwelt: Technik
• ISBN-10: 1-4832-7235-4 / 1483272354
• ISBN-13: 978-1-4832-7235-1 / 9781483272351