Molecular Breeding and Genetics of Applied Microorganisms (eBook)

Masanori Okanishi, Kenji Sakaguchi (Herausgeber)

eBook Download: PDF

2013 | 1. Auflage
172 Seiten
Elsevier Science (Verlag)
978-1-4832-7033-3 (ISBN)

Infos

Molecular Breeding and Genetics of Applied Microorganisms provides expert reviews on the developments in the field of applied microbiology in Japan. The book presents areas where further progress in applied microbiology is possible. Articles on these possibilities such as the use of recombinant DNA techniques to link fragments of DNA from unrelated organisms; creation of new strains of microorganisms to control pollution; construction of a ''biological battery'' using a photosynthetic system; and breeding of plant species tolerant to various plant diseases are explored in detail. Microbiologists, biologists, geneticists, biochemists, pharmacologists, and researchers will find the book insightful.

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Molecular Breeding and Genetics of Applied Microorganisms provides expert reviews on the developments in the field of applied microbiology in Japan. The book presents areas where further progress in applied microbiology is possible. Articles on these possibilities such as the use of recombinant DNA techniques to link fragments of DNA from unrelated organisms; creation of new strains of microorganisms to control pollution; construction of a "e;"e;biological battery"e;"e; using a photosynthetic system; and breeding of plant species tolerant to various plant diseases are explored in detail. Microbiologists, biologists, geneticists, biochemists, pharmacologists, and researchers will find the book insightful.

Inhalt

Front Cover 1
Molecular Breeding and Genetics of Applied Microorganisms 4
Copyright Page 5
Table of Contents 6
Contributors 8
Preface 10
Introduction 12
Chapter 1. Species Barriers to the Maintenance and Expression of Foreign DNA 14
1.1 Barriers to the Genetic Establishment and Expression of Foreign DNA 15
1.2 Selective Disadvantage of Hosts Harboring Reeombinant Molecules 19
REFERENCES 19
Chapter 2. Regulation of Antibiotics Production in Microorganisms 22
2.1 Regulation of the Initiation of Secondary Metabolism 23
2.2 Characteristics of Enzymes Involved in Secondary Metabolism 32
2.3 Quantitative Regulation of Antibiotics Production 35
REFERENCES 39
Chapter 3. Plasmid Involvement in Antibiotics Production 42
3.1 The Nature of Secondary Metabolites 42
3.2 Evidence of Plasmid Involvement 43
3.3 Plasmid Involvement in Chloramphenicol Production 46
3.4 Plasmid Involvement in Aureothricin Production 51
3.5 Plasmid DNA in Streptomycetes 55
3.6 Plasmid Function in Antibiotics Production 57
REFERENCES 59
Chapter 4. Degradative Plasmids: Aspect of Microbial Evolution 60
4.1 Introduction 60
4.2 Physiology of Degradative Plasmids 61
4.3 Phenotype of Degradative Plasmids 65
4.4 Handling of Degradative Plasmids 68
4.5 Adaptation and Evolution 70
4.6 Closing Remarks 71
REFERENCES 71
Chapter 5. Selection of Genes with Antibodies against the Gene Products 74
5.1 Purification of Calf Rennin mRNA 74
5.2 Bovine Corticotropin—ß-Lipotropin Precursor 81
REFERENCES 85
Chapter 6. Enzymatic Synthesis of Polynucleotides of Defined Sequence 88
6.1 Polynucleotide Phosphorylase 88
6.2 RNA Ligase 91
Chapter 7. Protoplast Fusion 98
7.1 Brevibacterium 98
7.2 Streptomyces 101
7.3 Saccharomyces 107
7.4 Aspergillus 116
REFERENCES 118
Chapter 8. Cloning with Recombinant DNA 120
8.1 Bacillus subtilis 120
8.2 Development of Molecular Cloning Systems in Pseudomonas 137
8.3 Highly Thermophilic Bacteria 140
8.4 Cloning of Streptomyces DNA in Escherichia coli 143
REFERENCES 150
Chapter 9. New Genetic Approaches to Industrial Microorganisms 152
9.1 Amino Acid-Producing Strains 152
9.2 Fermentative Production of "Magic Spots" 160
REFERENCES 166
Subject Index 168
Index of Microorganisms 172

Produkt-Details

• Erscheint lt. Verlag: 22.10.2013
• Sprache: englisch
• Themenwelt: Naturwissenschaften ► Biologie ► Evolution
• Themenwelt: Technik
• ISBN-10: 1-4832-7033-5 / 1483270335
• ISBN-13: 978-1-4832-7033-3 / 9781483270333