Thermal and Energetic Studies of Cellular Biological Systems (eBook)

A. M. James (Herausgeber)

eBook Download: PDF

2016 | 1. Auflage
232 Seiten
Elsevier Science (Verlag)
978-1-4831-9355-7 (ISBN)

Infos

Thermal and Energetic Studies of Cellular Biological Systems reviews literature on thermal and energetic changes which occur in living organisms. It was commissioned at the suggestion of Dr. Philip Edge of John Wright & Sons and was a natural successor to the 1980 book Biological Microcalorimetry. This volume is restricted to a discussion of energy changes in cellular systems. This book is organized into nine chapters. Each author presents a concise, up-to-date account of his field of expertise. Their topics include the usefulness of calorimetric methods in ecological studies, growth and metabolism in bacteria and yeasts, metabolism and heat dissipation in whole tissues or organs, and animal cells and energy requirements in biological systems. This book will be of interest to people seeking a non-destructive technique for studying cellular system and it can serve as a guide and a reference book to those already active in the field.

Inhalt

Front Cover 1
Thermal and Energetic Studies of Cellular Biological Systems 4
Copyright Page 5
Preface 6
Table of Contents 10
Contributors 8
Chapter 1. Calorimetry, Past,Present, Future 12
1.1 Historical 12
1.2 Reporting Experimental Results 13
1.3 Provisional Recommendations for the Measurement and Presentation of Results [ IUPAC, IUPAP and IUB 1982, reproduced with permission from Pure and Applied Chemistry ( 1982), 54,676–9] 17
1.4 Applications of Calorimetrie Studies 23
1.5 Future of Calorimetry in the Study of Cellular Biological Systems 23
References 24
Chapter 2. Energy Requirements in Biological Systems 25
2.1 Introduction 26
2.2 Theory 26
2.3 Efficiency of Biological Systems 31
2.4 Maintenance Energy Requirements 40
2.5 Heat of Fermentation 41
2.6 Summary 42
A cknowledgement 42
References 42
Chapter 3. Calorimetrie Techniques 45
3.1 Introduction 45
3.2 Some Calorimetrie Principles and Concepts 45
3.3 Some Calorimetrie Designs 52
3.4 Systematic Errors and Other Problems 71
References 76
Chapter 4. Growth and Metabolism of Bacteria 79
4.1 Introduction 79
4.2 Estimation of the Enthalpy of Metabolism 80
4.3 Experimental Arrangements for Monitoring Heat Production 81
4.4 Interpretation of Thermal Data 85
4.5 Applications of Thermal Studies 110
References 112
Chapter 5. Growth and Metabolism of Yeasts 117
5.1 Introduction 117
5.2 Microcalorimetric Studies of Yeast Growth/Metabolism 119
5.3 Commercial Applications 132
5.4 Concluding Remarks 137
References 138
Chapter 6. In Vitro Thermal Studies of Blood Cells 142
6.1 Introduction 142
6.2 Calorimeters 142
6.3 Erythrocytes 143
6.4 Platelets or Thrombocytes 147
6.5 Lymphocytes 149
6.6 Granulocytes or Polymorphonuclear Neutrophils (PMNs) 152
6.7 Leukocyte Mixtures 154
6.8 Whole Blood 155
References 156
Chapter 7. Heat Dissipation and Metabolism in Isolated Animal Cells and Whole Tissues/Organs 158
7.1 Introduction 158
7.2 Long Term Studies of Cellular Heat Production 159
7.3 Short Term Thermograms and the Effects of Diverse Agents 163
7.4 Combination of Heat Measurements with Biochemical Assays 168
7.5 Conclusions 175
References 176
Chapter 8. Transfer Thermodynamics 178
8.1 Introduction 179
8.2 Background 179
8.3 Thermodynamic Aspects 180
References 191
Chapter 9. Application of Calorimetrie Methods in Ecological Studies 193
9.1 Introduction 193
9.2 Microcalorimetric Methods 195
9.3 Microbial Ecology 197
9.4 Aerobic and Anoxic Metabolism in Aquatic Invertebrates and Fish 202
9.5 Toxicological and Pharmacological Applications 222
9.6 Conclusions 223
Acknowledgements 224
References 224
Index 228

Produkt-Details

• Erscheint lt. Verlag: 29.7.2016
• Sprache: englisch
• Themenwelt: Naturwissenschaften ► Biologie ► Zellbiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Zoologie
• Themenwelt: Technik
• ISBN-10: 1-4831-9355-1 / 1483193551
• ISBN-13: 978-1-4831-9355-7 / 9781483193557