Blick ins Buch

Plant Cell Expansion

Methods and Protocols

José M. Estevez (Herausgeber)

Buch | Hardcover

231 Seiten

2014
Humana Press Inc. (Verlag)
978-1-4939-1901-7 (ISBN)

Lese- und Medienproben

Blick ins Buch (midvox)

Artikel merken

This volume covers broad aspects of cell expansion in three different cell types: root hairs, pollen tubes, and hypocothyl cells. Chapters focus on the cutting-edge methods to study in detail several complex aspects of cell expansion such as secretion, endocytosis and recycling, cellular signaling and trafficking, and protein and polysaccharides cell wall biosynthesis in real time during cell expansion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Cell Expansion: Methods and Protocols is an essential reference book for plant scientist, molecular, and cell biologist as well as plant biochemists.

Penium margaritaceum as a Model Organism For Cell Wall Analysis Of Expanding Plant Cells.- Using Chemical Genomics to Study Cell Wall Formation and Cell Growth in Arabidopsis thaliana and Penium margaritaceum.- Optimized Method For Growing In Vitro arabidopsis Thaliana Pollen Tubes.- Imaging of Calcium Dynamics in Pollen Tube Cytoplasm.- Live Imaging of Root Hairs.- Improved ROS Measurement In Root Hair Cells.- A Root Hair Assay to Expedite Cell Death Research.- Vacuolar Staining Methods in Plant Cells.- Live Cell Imaging Of FM4-64, A Tool For Tracing The Endocytic Pathways In Arabidopsis Root Cells.- Salt-Stress Regulation Of Root System Growth And Architecture In Arabidopsis Seedlings.- Quantification Of Fluorescent Reporters in Plant Cells.- Live Cell Imaging Of The Cytoskeleton And Cell Wall Enzymes In Plant Cells.- Using the Split-Ubiquitin Yeast Two-Hybrid System to Test Protein-Protein Interactions of Transmembrane Proteins.- Activation Tag Screening For Cell Expansion Genes in Arabidopsis thaliana.- Bifc For Protein-Protein Interactions and Protein Topology: Discussing An Integrative Approach For An Old Technique.- N-Glycosylation and Plant Cell Growth.- Peptide Separation Methodologies for In-depth Proteomics.- Structural And Mechanical Characterization Of Growing Arabidopsis< Plant Cell Walls.

Reihe/Serie	Methods in Molecular Biology ; 1242
Zusatzinfo	41 Illustrations, color; 8 Illustrations, black and white; XI, 231 p. 49 illus., 41 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Botanik
Schlagworte	Cell expansion • cell wall biosynthesis • hypocotyls • Penium margaritaceum • plant cell expansion • Plant Cells • pollen tubes • root hairs • single-cell growth • whole roots
ISBN-10	1-4939-1901-6 / 1493919016
ISBN-13	978-1-4939-1901-7 / 9781493919017
Zustand	Neuware