Diagnostic Cytogenetics

Buch | Softcover

XIV, 460 Seiten

2014
Springer Berlin (Verlag)
978-3-642-47813-0 (ISBN)

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In addition to the traditional cytogenetics still used as the basic methodology for everyday clinical diagnosis, new molecular cytogenetic techniques provide a useful basis for routine diagnosis. Flourescence in situ hybridization (FISH) has become a standard technique, and comparative genomic hybridization (CGH), spectral karyotyping (SKY), and multi-color FISH have shown their potential for diagnostic purposes. Following a section on tissue culture, chromosome staining and basic information about karyotyping, nomenclature and quality standards, protocols of relevance for comprehensive cytogenetic diagnostics are presented.

I Classical Cytogenetics.- 1 Tissue Culture.- 2 Chromosome Staining.- 3 Karyotyping and Data Interpretation.- 4 Documentation.- II Postnatal Diagnosis.- 5 Peripheral Blood.- 6 Establishment of Permanent Growing Lymphoblastoid Cell Lines.- 7 Solid Tissues.- 8 Cells from Urine Sample.- 9 Classical and Molecular Cytogenetics of Tumor Cells.- 10 Cytogenetics of Meiotic Cells.- III Prenatal Diagnosis.- 11 Prenatal Diagnosis - An Introduction.- 12 Amniotic Fluid Cell Analysis.- 13 Chorionic Villi Sampling.- IV Special Applications.- 14 Diagnosis of Chromosomal Instability Syndromes.- 15 Flow Cytometric Testing for Syndromes with Chromosomal Instability, Aplastic Anemia and Related Hematological Disorders.- 16 Cell Fusion.- 17 Origin of Trisomies.- V Molecular Cytogenetics.- 18 Fluorescence in Situ Hybridization.- 19 Fluorescence in Situ Hybridization (FISH) Analysis in Human Sperm Cells.- 20 Microdissection and Reverse Chromosome Painting.- 21 Comparative genomic hybridisation (CGH).- VI Techniques in Development.- 22 Fetal Cells in Maternal Blood.- 23 Spectral Karyotyping in Clinical and Tumor Cytogenetics.- 24 Chromosome Analysis by Multiplex-FISH (M-FISH).

Erscheint lt. Verlag	15.4.2014
Reihe/Serie	Springer Lab Manuals
Zusatzinfo	XIV, 460 p. 66 illus., 7 illus. in color.
Verlagsort	Berlin
Sprache	englisch
Maße	203 x 254 mm
Gewicht	965 g
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	Cell • Chromosom • chromosome • Chromosomen • Chromosomes • cytogenetics • Cytogenetik • Fluorescence in situ hybridisation • Fluoreszentz-in-situ-Hybridisierung • Fluoreszenz in situ-Hybridisierung • Genetics • Genome • Hybridization • karyotyping • Pränatale Diagnose • Prenatal Diagnosis • tissue • Tumor cells
ISBN-10	3-642-47813-1 / 3642478131
ISBN-13	978-3-642-47813-0 / 9783642478130
Zustand	Neuware