Stem Cells and Small RNAs
Academic Press Inc (Verlag)
978-0-12-800651-1 (ISBN)
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Contents
Part I: Small RNAs
1. Outline
2. Rational design and high efficiency of siRNAs and shRNAs
Molecular structures of small RNAs
Working mechanisms of sRNAs
Essential factors of rational design
Biological traits of sRNAs with high efficiency
Gene regulation of endogenous sRNAs
3. Side-effects of siRNA and shRNA
Off-targets
Identification of off-target effect
Immune stimulatory effects
Toxic effects of sRNA
4. miRNAs regulating noncoding and coding mRNAs
Working principles of miRNAs
Regulation of miRNA expression
Discovery of ALU-based miRNAs
Function of ALU-based miRNAs
miRNA drugs
5. Discovery and functions of endogenous short-hairpin RNAs
Discovery of endogenous shRNAs in human
Silencing models of endogenous shRNAs
Physiological function of endogenous shRNA
Difference between endogenous shRNAs and miRNAs
Evolution of shRNAs
6. Transfection of siRNAs into floating cells or stem cells
Current issues of delivery systems
Liposome-based transfection reagents
Polymer-based veihcal
Virus -based transduction methods
Peptide nanoparticals
7. Transdermal delivery of small RNAs in vivo
Injection of Nude siRNAs
Cholesterol-conjugated siRNAs
Transdermal and Mucusal delivery
Inhalation aerosols
Stable Nucleic Acid Lipid Particle (SNALP)
8. sRNAs and stem cells
sRNA-omics of stem cells
Regulation of stem cell self-renewal by sRNAs
sRNA-mediated modulation on stem cell differentiation
9. siRNA gene drugs
RNAi-mediated validation of genes as drug targets
Special traits of siRNA-based drugs
Clinical trials of siRNA drugs
Development of siRNA drugs in China
Part II: Stem Cells
1. Oultines
2. Embryonic stem cells
Derivation of human embryonic stem cell lines
Phenotypic analysis of human ESCs
Genetic and epigenetic analysis of human ESCs
ESC self-renewal and differentiation
RNAi in ESCs
3. Adult stem cells
Isolation and identification of MSCs from different sources
Large-scale expansion of MSCs
Identification and characterization of MSCs
Senescence of MSCs
Immuno-modulation and homing of MSCs
Genetically engineering MSCs
4. Induced pluripotent stem cells
Concept of reprogramming
Phenotypic analysis of human iPSCs
Genetic and epigenetic traits of human iPSCs
Molecular mechanisms of reprogramming
Human iPSC-based disease model for drug discovery
5. Trans-determination of stem cells
Conversion of MSCs to neural stem cells
Differentiation of MSCs to Cardiac stem cells
Conversion of MSCs to endothelial stem cells
Hepatogenic differentiation of MSCs
Transformation of MSCs to pancreatic stem cells
6. Induced hematopoietic stem cells
Conversion of MSCs into HSCs
Immunocytochemical analysis of human stem cells
Analysis of genome-wide gene expression profiles
In vitro colony-forming assay
In vivo reconstitution of marrow functions
Classical cytogenetics: karyotyping
7. Cancer stem cells
Isolation and characterization of CSCs
Enrichment and culture of CSCs
Molecular basis of CSC resistance
Migration of CSCs
Signaling networks of CSCs
8. Molecular mechanisms of trans-determination
Concept of transdetermination state
Genetic and epigenetic analysis of transdetermination
Difference between reprogramming, de-differentiating and transdetermining
Signaling networks of transdetermination
Prospective importance of transdetermination
9. Stem cell drugs in China
Concept of stem cell drugs
R&D of Stem cell drugs
Basic facilities and conditions
Clinical studies
Regulation of stem cell drugs
Part III three: Methods
1. Isolation, characterization and maintenance of MSCs
2. Approaches for derivation and expansion of HSCs
3. Activation of MSC transdetermination state
4. Bioinformatics of transdetermination
Microarray approach to identify signaling pathways responsible for transdetermination
5. Approaches for enrichment, characterization, and maintenance of cancer stem cells
6. Technologies to generate iPS cells
Genetic engneering
mRNA inducing methods
miRNAs
Small molecules
6. Microarrays of small RNAs
7. Molecular sensors detecting small RNAs
8. Micro-analysis of stem cell biological trails
9. Gene-modified stem cells
10. Clinical grade generation of stem cells
Part IV: Application
1. Outlines
2. siRNA-based de-pigmenting and brightening cosmetics
3. siRNA treatment in psoriases model
4. miRNA and heart disease
5. MSC-derived HSCs transplantation
6. MSC-derived RBCs for fabrication of blood
7. MSC-derived RBCs for generation of platelets
8. MSC-derived RBCs for production of CIK cells
9. MSC-derived neural stem cells for cerebral palsy
10. Anti-aging application of MSCs
11. MSC-derived pancreatic stem cells and diabetes
| Erscheint lt. Verlag | 13.5.2016 |
|---|---|
| Verlagsort | San Diego |
| Sprache | englisch |
| Maße | 152 x 229 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| ISBN-10 | 0-12-800651-X / 012800651X |
| ISBN-13 | 978-0-12-800651-1 / 9780128006511 |
| Zustand | Neuware |
| Informationen gemäß Produktsicherheitsverordnung (GPSR) | |
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