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Aqueous Size-exclusion Chromatography

Buch | Hardcover

472 Seiten

1988
Elsevier Science Ltd (Verlag)
978-0-444-42957-5 (ISBN)

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The rapid development of new packings for aqueous size-exclusion chromatography has revolutionized this field. High resolution non-adsorptive columns now make possible the efficient separation of proteins and the rapid and precise determination of the molecular weight distribution of synthetic polymers. This technology is also being applied to the separation of small ions, the characterization of associating systems, and the measurement of branching. At the same time, fundamental studies are elucidating the mechanisms of the various chromatographicprocesses. These developments in principles and applications are assembled for the first time in this book. Fundamental issues are dealt with: the roles of pore structure and macromolecular dimensions, hydrophobic and electrostatic effects, and the determination and control of column efficiency. High-performance packings based on derivatized silica are reviewed in detail. Special techniques are thoroughly described, including SEC/LALLS, inverse exclusion chromatography, and frontal zone chromatography.

Part I. Separation Mechanisms. Size exclusion parameters (M.E. Himmel, P.G. Squire). Partitioning: Hydrophobic interactions (M. Janado). Electrostatic effects (P.L. Dubin). Exclusion chromatography of inorganic compounds (M. Shibukawa, N. Ohta). II. Characterization of Stationary Phases. Pore size distributions (L. Hagel). Structural analysis of porous materials by measurement of size exclusion (S. Kuga). Column efficiency (S. Mori). III. New Packings. Native and bonded silicas in aqueous SEC (K.K. Unger, J.N. Kinkel). Rigid polymer gels for SEC and their application to biopolymers (K. Makino, H. Hatano). IV. Biopolymers. Biopolymers. I. Protein chromatography in denaturing and non-denaturing solvents (R.C. Montelaro). Biopolymers. II. Serum lipoproteins (M. Okazaki, I. Hara). Application of SEC/LALLS to biopolymer assemblies (K. Konishi). V. Associating Systems. Measurement of equilibrium constants by exclusion methods (T.K. Korpela, J.-P. Himanen). 14. Frontal boundary analysis in size exclusion chromatography of self-associating proteins (G.W. Becker). 15. Exclusion chromatography of micelles (K.S. Birdi). Subject Index.

Reihe/Serie	Journal of Chromatography Library
Verlagsort	Oxford
Sprache	englisch
Maße	150 x 230 mm
Themenwelt	Naturwissenschaften ► Chemie ► Analytische Chemie
ISBN-10	0-444-42957-3 / 0444429573
ISBN-13	978-0-444-42957-5 / 9780444429575
Zustand	Neuware