Plant Molecular Biology Manual

S.B. Gelvin, R.A. Schilperoort (Herausgeber)

Buch | Softcover

161 Seiten

2012 | Second Edition 2000
Springer (Verlag)
978-94-011-7655-2 (ISBN)

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Plant Molecular Biology Manual (Second Edition) is an entirely new manual containing both fundamental and recently described techniques in the area of plant molecular biology. Designed for use in the research laboratory, the Plant Molecular Biology Manual presents detailed techniques in the areas of plant transformation, recombinant DNA and other nucleic acid manipulations, nuclear run-on and in vitro transcription systems, in situ hybridization and immunodetection systems, protein-nucleic acid interaction analyses, subcellular targeting of proteins in the plant cell, and gene tagging using T-DNA and transposons. This second edition contains more than 40 newly written chapters, including descriptions of subjects such as virus-mediated gene transfer, specialized Agrobacterium strains and T-DNA vectors, nuclear run-on and in vitro transcription systems, non-radioactive detection systems, characterization of transcription factors, nuclear protein targeting, and T-DNA and transposon mutagenesis, not previously described in the first edition.

E3: Yeast as versatile tool in transcription factor research.- H5: The construction of bacterial artificial chromosome (BAC) libraries.- N1: Gene transfer mediated by site-specific recombination systems.- N2: Use of the R-RS site-specific recombination system in plants.- N3: Cre-lox directed integration of transgenes into the tobacco genome.- 01: Plant actin isolation and characterization.

Zusatzinfo	XXI, 161 p.
Verlagsort	Dordrecht
Sprache	englisch
Maße	155 x 235 mm
Themenwelt	Naturwissenschaften ► Biologie ► Botanik
ISBN-10	94-011-7655-8 / 9401176558
ISBN-13	978-94-011-7655-2 / 9789401176552
Zustand	Neuware