Flower Development
Humana Press Inc. (Verlag)
978-1-4614-9407-2 (ISBN)
In Flower Development: Methods and Protocols, researchers in the field detail protocols for experimental approaches that are currently used to study the formation of flowers, from genetic methods and phenotypic analyses, to genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls
Authoritative and practical, Flower Development: Methods and Protocols is an essential guide for plant developmental biologists, from the novice to the experienced researcher, and for those considering venturing into the field.
Flower Development in Arabidopsis - There is More to it than Learning your ABCs.- Flower Development in the Asterid Lineage.- Grass Flower Development.- Flower Diversity and Angiosperm Diversification.- Flower Development - Open Questions and Future Directions.- Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors.- Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development.- Genetic and Phenotypic Analyses of Petal Development in Arabidopsis.- Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and Rice.- Molecular Cell Biology of Male Meiotic Chromosomes and Isolation of Male Meiocytes in Arabidopsis thaliana.- Genetic and Phenotypic Analyses of Carpel Development in Arabidopsis.- Microscopic Analysis of Arabidopsis Ovules.- Scanning Electron Microscopy Analysis of Floral Development.- Detection of mRNA Expression Patterns by Non-radioactive in situ Hybridization on Histological Sections of Floral Tissue.- The GUS Reporter System in Flower Development Studies.- A Floral Induction System for the Study of Early Arabidopsis Flower Development.- Fluorescence Activated Cell Sorting of Shoot Apical Meristem Cell Types.- Translating Ribosome Affinity Purification (TRAP) for Cell-specific Translation Profiling in Developing Flowers.- Laser-assisted Microdissection Applied to Floral Tissues.- Identification of Arabidopsis Knock-out Lines for Genes of Interest.- Gene Expression Analysis by Quantitative Real-time PCR for Floral Tissues.- Misexpression Approaches for the Manipulation of Flower Development.- Next Generation Sequencing Applied to Flower Development: RNA-Seq.- Next-Generation Sequencing Applied to Flower Development: ChIP-Seq.- Live-Imaging of the Arabidopsis Inflorescence Meristem.- Gene Regulatory Network Models for Floral Organ Determination.
| Reihe/Serie | Methods in Molecular Biology ; 1110 |
|---|---|
| Zusatzinfo | 31 Illustrations, color; 33 Illustrations, black and white; XIV, 475 p. 64 illus., 31 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Botanik |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| ISBN-10 | 1-4614-9407-9 / 1461494079 |
| ISBN-13 | 978-1-4614-9407-2 / 9781461494072 |
| Zustand | Neuware |
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