Developmental Biology Protocols

Volume I

Rocky S. Tuan, Cecilia W. Lo (Herausgeber)

Buch | Hardcover

571 Seiten

1999 | 2000 ed.
Humana Press Inc. (Verlag)
978-0-89603-852-3 (ISBN)

Artikel merken

Developmental biology is one of the most exciting and fast-growing fields - day. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the organism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and molecular biology, as well as evolutionary biology. No longer is the study of embryonic development merely "embryology." In fact, development biology has produced important paradigms for both basic and clinical biomedical sciences alike. Though modern developmental biology has its roots in "experimental embry- ogy" and the even more classical "chemical embryology," the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the "cellular and molecular biology revolution." The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

Developmental Biology Protocols.- Systems: Production, Culture, and Storage.- Rearing Larvae of Sea Urchins and Sea Stars for Developmental Studies.- Large-Scale Culture and Preparation of Sea Urchin Embryos for Isolation of Transcriptional Regulatory Proteins.- The Chick Embryo as a Model System for Analyzing Mechanisms of Development.- Culture of Avian Embryos.- Exo Ovo Culture of Avian Embryos.- Culture of Preimplantation Mouse Embryos.- In Vitro Culture of Rodent Embryos During the Early Postimplantation Period.- Cryopreservation of Mouse Embryos.- Developmental Pattern and Morphogenesis.- Studying Head and Brain Development in Drosophila.- Bioassays of Inductive Interactions in Amphibian Development.- Gastrulation and Early Mesodermal Patterning in Vertebrates.- Craniofacial Development of Avian and Rodent Embryos.- Examination of the Axial Skeleton of Fetal Rodents.- Cardiac Morphogenesis and Dysmorphogenesis.- Embryo Structure and Function.- Application of Plastic Embedding for Sectioning Whole-Mount Immunostained Early Vertebrate Embryos.- Confocal Microscopy of Live Xenopus Oocytes, Eggs, and Embryos.- Whole-Mount Immunolabeling of Embryos by Microinjection.- Confocal Laser Scanning Microscopy Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos.- Embryo/Fetal Topographical Analysis by Fluorescence Microscopy and Confocal Laser Scanning Microscopy.- Magnetic Resonance Imaging Analysis of Embryos.- Optical Coherence Tomography Imaging in Developmental Biology.- Ultrasound Backscatter Microscopy of Mouse Embryos.- Use of Doppler Echocardiography to Monitor Embryonic Mouse Heart Function.- Calcium Imaging in Cell-Cell Signaling.- Acquisition, Display, and Analysis of Digital Three-Dimensional Time-Lapse (Four-Dimensional) Data Sets Using Free Software Applications.- Cell Lineage Analysis.- Cell Lineage Analysis.- Cell Lineage Analysis.- Retroviral Cell Lineage Analysis in the Developing Chick Heart.- Dynamic Labeling Techniques for Fate Mapping, Testing Cell Commitment, and Following Living Cells in Avian Embryos.- Cell Lineage Analysis.- Cell Lineage Analysis in Xenopus Embryos.- Photoactivatable (Caged) Fluorescein as a Cell Tracer for Fate Mapping in the Zebrafish Embryo.- Carboxyfluorescein as a Marker at Both Light and Electron Microscope Levels to Follow Cell Lineage in the Embryo.- Chimeras.- Transplantation Chimeras.- Interspecific Chimeras in Avian Embryos.- Quail-Chick Transplantation in the Embryonic Limb Bud.- Mouse Chimeras and the Analysis of Development.- Cell Grafting and Fate Mapping of the Early-Somite-Stage Mouse Embryo.- Interspecific Chimeras.- Interspecific Mouse-Chick Chimeras.- Mosaic Analysis in Caenorhabditis elegans.- Experimental Manipulation of Embryos.- Local Application of Bone Morphogenic Protein on Developing Chick Embryos Using a Fibrous Glass Matrix as a Carrier.- Laser Ablation and Fate Mapping.- Photoablation of Cells Expressing ?-Galactosidase.- Exo utero Surgery.- Application of Viral Vectors in the Analysis of Development.- Methods for Constructing and Producing Retroviral Vectors.- Retroviral Gene Transduction in Limb Bud Micromass Cultures.- Construction of Adenoviral Vectors.- Application of Adenoviral Vectors.- The Application of Adenoviral Vectors in the Study of Mammalian Cardiovascular Development.

Erscheint lt. Verlag	19.11.1999
Reihe/Serie	Methods in Molecular Biology ; 135
Zusatzinfo	XXVIII, 571 p.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
ISBN-10	0-89603-852-1 / 0896038521
ISBN-13	978-0-89603-852-3 / 9780896038523
Zustand	Neuware