Pyrosequencing Protocols

Buch | Softcover

196 Seiten

2010 | 1st ed. Softcover of orig. ed. 2007
Humana Press Inc. (Verlag)
978-1-61737-693-1 (ISBN)

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Pyrosequencing® Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing® technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each protocol with troubleshooting tips specific to that method.

Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing® methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing® technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing® comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing®.

The History of Pyrosequencing®.- Pyrosequencing® Applications.- Web-Based Primer Design Software for Genome-Scale Genotyping by Pyrosequencing®.- Pyrosequencing®.- Universal Primer Applications for Pyrosequencing®.- Allele Quantification and DNA Pooling Methods.- Multiplex Pyrosequencing® for DNA Variation Analysis.- Analysis of Gene-Specific DNA Methylation Patterns by Pyrosequencing® Technology.- Detecting Mutations That Confer Oxazolidinone Resistance in Gram-Positive Bacteria.- Pyrosequencing®-Based Strategies for Improved Allele Typing of Human Leukocyte Antigen Loci.- Pyrosequencing® of Phage Display Libraries for the Identification of Cell-Specific Targeting Ligands.- Gene Copy Number Detection in Animal Studies.- Detection of Allelic Imbalance in Gene Expression Using Pyrosequencing®.- Pyrosequencing® Genotype Storage Techniques.

Erscheint lt. Verlag	1.12.2010
Reihe/Serie	Methods in Molecular Biology ; 373
Zusatzinfo	XII, 196 p.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	152 x 229 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
	Naturwissenschaften ► Biologie ► Biochemie
ISBN-10	1-61737-693-0 / 1617376930
ISBN-13	978-1-61737-693-1 / 9781617376931
Zustand	Neuware