ADAMTS Proteases

Methods and Protocols

X. Long Zheng (Herausgeber)

Buch | Hardcover

350 Seiten

2026
Humana (Verlag)
978-1-0716-5217-6 (ISBN)

Infos

This volume provides methods for the analysis of ADAMTS proteases and ADAMTS-like proteins. Chapters detail ADAMTS family members, with special attention to the exciting progress made in understanding ADAMTS13—its biology, disease mechanisms, detection, and clinical management of a potentially fatal thrombotic microangiopathy, thrombotic thrombocytopenic purpura (TTP). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, ADAMTS Proteases: Methods and Protocol is a valuable resource for any researcher hoping to contribute to the ever expanding world of ADAMTS.

Inhalt

An overview of ADAMTS proteases.- Overview of the Roles of ADAMTS1 in Ovarian Biology.- Methods for Defining the Role of ADAMTS1 in the Ovary.- Modeling Aspects of Human Placental Development with Trophoblast Stem Cells.- Exploring The Role of ADAMTS4 Expression in Atherosclerosis.- Method for Generation of adamtsl4 knock-out zebrafish lines using CRRISPR/Cas9 system.- Assay for neurocan-degrading activity of  ADAMTS12  metalloprotease.- Generating adamts13-/- Zebrafish via CRISPR/Cas9 Gene Editing.- Determining ADAMTS13 conformation for the diagnosis of immune thrombotic thrombocytopenic purpura and prediction of relapse.- Methods for studying the role of ADAMTS13 in angiogenesis.- Assays for Plasma ADAMTS13 Activity and Inhibitors using recombinant FRETS-VWF73 peptide.- A Vortex-Shear Based Assay for Cleavage of Multimeric VWF by ADAMTS13.- Deep protease profiling to define the substrate specificity of ADAMTS proteases.- Microfluidic shear-based assay for assessing ADAMTS13 activity under flow- Optical‑Tweezers Single‑Molecule Assay for ADAMTS13‑Mediated Proteolysis of the von Willebrand Factor A2 Domain.- Method for Assessing Inhibition Kinetics of Autoantibodies Against ADAMTS13 in Immune Thrombotic Thrombocytopenic Purpura.- Hydrogen Exchange Mass Spectrometry for Mapping Antibody Binding Sites and Conformational Dynamics of ADAMTS13.- Treating Immune Thrombotic Thrombocytopenic Purpura with Therapeutic Plasma Exchange.- Molecular Dynamics Simulations with NAMD and Trajectory Analysis.- Treating immune-mediated thrombotic thrombocytopenic purpura by Caplacizumab.- Treating Immune Thrombocytopenic Purpura by Immunosuppressive Agents.

Produkt-Details

• Erscheint lt. Verlag: 29.5.2026
• Reihe/Serie: Methods in Molecular Biology
• Zusatzinfo: 5 Illustrations, color; 48 Illustrations, black and white
• Sprache: englisch
• Maße: 178 x 254 mm
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie
• Themenwelt: Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
• Schlagworte: atherosclerosis • Cancer Biology • CRISPR • immunology • Mass Spectrometry • metalloprotinases
• ISBN-10: 1-0716-5217-6 / 1071652176
• ISBN-13: 978-1-0716-5217-6 / 9781071652176
• Zustand: Neuware