Fluorescence In Situ Hybridization (FISH)
Humana (Verlag)
9781071651490 (ISBN)
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Authoritative and cutting-edge, Fluorescence In Situ Hybridization (FISH): Application Guide, Thid Edition aims to be useful to researchers just starting to study FISHers and for FISH experts.
Background.- Microscopy and imaging: Visualization matters.- Optical filters and light sources for FISH.- Automation of FISH-procedure and evaluation.- Classification of FISH probes.- Commercial FISH probes.- Generation of painting probes from flow-sorted and microdissected chromosomes.- FISH-microdissection.- Homemade locus-specific FISH probes: Bacterial artificial chromosomes.- The standard FISH procedure.- Microwave treatment for better FISH results in a shorter time.- FISH with and without COT DNA.- One-day FISH procedure.- Quantitative FISH (Q-FISH).- Telomere length measurement by FISH: Image processing techniques.- Directional Genomic Hybridization (dGH) for high-resolution detection of intrachromosomal rearrangements.- The replicative detargeting FISH (ReD-FISH) technique in studies of telomere replication.- Pre- and postnatal diagnostics and research on peripheral blood, bone marrow chorion, amniocytes, and fibroblasts.- Application of FISH to previously GTG-banded and/or embedded cytogenetic slides.- FISH in native amniocytes.- Tumorcytogenetic diagnostics and research on blood and bone marrow smears or effusions.- Characterization of mosaicism in different easy-to-acquire body tissues such as buccal smears, skin abrasions, hair root cells or urine.- Characterization of archived formalin-fixed/paraffin-embedded or cryofixed tissue, including nucleus extraction.- FISH on sperm, spermatocytes and oocytes.- Fluorescence in situ hybridization on DNA fibers: Molecular combing.- Two- to three-color FISH.- Multiplex FISH and spectral karyotyping .- FISH banding techniques.- Centromere directed M-FISH approaches (cenM-FISH).- Heterochromatin directed M-FISH (HCM-FISH).- FISH for repeatedly observed euchromatic variants.- Subtelomeric and/or subcentromeric probe sets.- Bar-coding is more actual than ever.- FISH for repeatedly observed constitutional rearrangements.- Parental origin determination FISH: Pod-FISH.- Simultaneous FISH and fluorescence immunostaining for DNA–protein co-detection.- CENP-antibodies used additionally to FISH.- X-inactivation accessed by FISH and EDU-immunostaining.- Immuno-FISH and microspreading technique for synaptonemal complex studies.- Yeast chromosomes revealed by immuno FISH.- Interphase FISH in diagnostics.- Interphase FISH for detection of chromosomal mosaicism and instability.- Comet-FISH.- Micronucleus FISH.- Three-dimensional interphase analysis using suspension FISH.- 3D DNA-FISH protocol for the study of the DUX gene in preimplantation bovine embryos.- Animal probes and Zoo-FISH.- Three-dimensional co-immuno and RNA-fluorescence in situ hybridization in preimplantation mouse embryos.- FISH in bird chromosomes: An updated protocol and best practices.- An updated standard protocol for FISH mapping in fish species (fish-FISH).- FISH in lampbrush-chromosomes.- General protocol for FISH on insect chromosomes.- FISH in Drosophila.- FISH on insect cells transfected with heterologous DNA – a single cell directed approach.- Fluorescence in situ hybridization for plants: Methods of chromosomal preparation.- Mitochondrial DNA (MtDNA) detection by FISH.- Advances in the application of FISH in food microbiology.- Evaluation of polymicrobial involvement using Fluorescence In Situ Hybridization (FISH) in clinical practice.- Comparative genomic hybridization (CGH) and microdissection-based CGH (micro-CGH) in human.- Comparative genomic hybridization (CGH) in animals.- Breakpoint mapping of balanced chromosomal rearrangements using array-CGH with microdissection-derived FISH probes.- Array CGH.- Chromosome microarray results to be verified by FISH.- Sequencing of microdissection derived FISH-probes.- Next generation sequencing should consider molecular cytogenetic results.- Optical genome mapping results need to be verified by FISH.- Application of ALFA-tag signal amplification to expand the CRISPR-based DNA imaging toolkit.
| Erscheint lt. Verlag | 4.4.2026 |
|---|---|
| Reihe/Serie | Springer Protocols Handbooks |
| Zusatzinfo | 76 Illustrations, color; 23 Illustrations, black and white |
| Verlagsort | New York |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| Schlagworte | Chromosomes • Comparative genomic hybridization • Fluorescence Microscopy • Immunostaining • microdissection • Molecular cytogenetic diagnostics • Multicolor FISH |
| ISBN-13 | 9781071651490 / 9781071651490 |
| Zustand | Neuware |
| Informationen gemäß Produktsicherheitsverordnung (GPSR) | |
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