T. cruzi Infection

Methods and Protocols

Karina Andrea Gómez, Carlos Andrés Buscaglia (Herausgeber)

Buch | Hardcover

445 Seiten

2025 | Second Edition 2026
Humana (Verlag)
9781071648476 (ISBN)

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This fully updated volume explores experimental methods to assess the complex interactions between Trypanosoma cruzi and its host and to study different aspects of T. cruzi and Chagas disease. The techniques presented, which touch on areas of bioinformatics, biochemistry, genetics, and cell biology research, have been recently developed or adapted to the T. cruzi system by leading experts in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and practical, T. cruzi Infection: Methods and Protocols, Second Edition provides a comprehensive understanding of the basic biology of this parasite and its crosstalk with the host immune response that aims to inspire further understanding of Chagas disease epidemiology, diagnosis, treatment, and clinical evaluation.

Computer-Aided Discovery of Drugs Against Chagas Disease.- Chromatin Interaction Maps of Trypanosoma cruzi.- GCanner, a Genome-Wide GC Composition Tool for the Unbiased Assessment of Trypanosoma cruzi Genomic Compartments.- An Algorithm for Annotation of Trypanosoma cruzi MASP Sequences Employing Hidden Markov Models.- Cloning-Free Genome Editing by CRISPR/T7RNAP/Cas9 in Trypanosoma cruzi.- Typification of Trypanosomatids Using Amplicon-Based Sequencing.- Deep Amplicon Sequencing of Minicircle Hypervariable Regions (mHVRs) for High-Resolution and Sensitive Trypanosoma cruzi Genotyping.- Identification of Trypanosoma cruzi Discrete Typing Units by Multiplex Real-Time PCR.- Real-Time PCR Assays for Quantification of Trypanosoma cruzi in Food Samples: A Tool for Surveillance of Oral Chagas Disease Outbreaks.- Ultra-Rapid DNA Extraction Coupled with Loop-Mediated Isothermal Amplification for Point-of-Care Detection of Trypanosoma cruzi Infection in Heparinized Blood and Dried Blood Spots.- Quantitative Real-Time PCR for Trypanosoma cruzi Infection Diagnosis and Treatment Response Monitoring of Patients with Chagas Disease.- Standardization of Serodiagnostic Methods for Trypanosoma cruzi.- Software and Tools to Analyze High-Density Peptide Array Data for the Chagas Antigen and Epitope Atlas.- Conjugation of Saccharidic Synthetic Antigens with Carrier Proteins by the Squaric Acid Method: Application as Biomarkers for Treatment Evaluation in Chagas Disease.- Identifying Trypanosoma cruzi Proteins Targeted by Chagas Disease Patient Antibodies Using Yeast Surface Display.- Profiling T-Cell Receptor and Immunoglobulin Repertoires through RNA-Sequencing to Understand Host Response to Trypanosoma cruzi Infection.- The Use of B-Cell Tetramers for the Study of Trypanosoma cruzi-Specific B-Cell Responses.- ELISPOT-Based Measurement of Antibody Secreting Cells and Memory B-Cells Specific to Trypanosoma cruzi in Chronically Infected Subjects.- Host-Derived Molecules as Novel Chagas Disease Biomarkers: Hypercoagulability Markers and Extracellular Vesicles.- Advanced Identification of Trypanosoma cruzi-Specific CD4+ and CD8+ T-Cells in Chronic Chagas Disease Patients Using Activation-Induced Marker (AIM) Assay.- Assessing the Metabolic Profile of CD4+ and CD8+ T-Lymphocytes in Chronic Chagas Disease Patients Using SCENITH.- A Single Cell Methodology for Energy Metabolism Analysis in Parasitic Protozoa.- Determining Histone Post-Translational Modifications along the Cell Cycle in Trypanosoma cruzi by Flow Cytometry.- Ultrastructure Expansion Microscopy for the Analysis of Trypanosoma cruzi Infection.- An In Vitro 3D Collagen-I Model for Studying Trypanosoma cruzi Trypomastigote Motility.- A Rapid Method for Purifying Intact Trypanosoma cruzi Amastigotes from Host Cells, In Vitro and In Vivo, for Downstream ‘Omic Analysis.- Insights into the Procedures and Interpretation of Autophagy in Trypanosoma cruzi.- Animal Models for Studying Trypanosoma cruzi Congenital Transmission and Associated Pathogenesis: Special Emphasis on Guinea Pig Model.

Erscheinungsdatum	06.11.2025
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	76 Illustrations, color; 15 Illustrations, black and white
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	Chagas Disease • Host Immune Response • in silico drug discovery • protozoan parasite • Serodiagnosis • Trypanosoma cruzi
ISBN-13	9781071648476 / 9781071648476
Zustand	Neuware