Human Respiratory Syncytial Virus

Methods and Protocols

Buch | Hardcover

377 Seiten

2025
Humana (Verlag)
978-1-0716-4665-6 (ISBN)

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This volume presents a comprehensive collection of state-of-the-art techniques used to investigate respiratory syncytial virus (RSV) at multiple levels, from atomic-resolution structural studies to in vivo infection models. It serves as an essential resource for virologists studying viral genome replication, transcription, and protein interactions, as well as immunologists exploring immune responses, pathogenesis, and vaccine development. Structural biologists will find valuable insights into the use of cryo-electron microscopy, X-ray crystallography, and other biophysical approaches to characterize viral components, while translational researchers and pharmaceutical scientists will benefit from protocols designed to facilitate the development of novel antivirals and vaccine candidates. Clinicians and epidemiologists studying RSV transmission, disease outcomes, and therapeutic interventions will also find this volume highly relevant to their work. As part of the renowned Methods in Molecular Biology series, this book follows a structured format, ensuring accessibility and reproducibility. Each chapter provides a concise introduction to the method’s background, relevance, and applications, followed by a detailed, step-by-step protocol accompanied by a comprehensive list of reagents, materials, and specialized equipment. Expert troubleshooting tips and insights are included to help optimize experimental success and avoid common pitfalls.

Bringing together contributions from leading researchers in the field, this volume offers both practical guidance and inspiration for future discoveries. Whether you are an experienced scientist or a newcomer to RSV research, Human Respiratory Syncytial Virus: Methods and Protocols provides the essential tools and knowledge to advance our understanding of RSV biology and accelerate the development of novel therapeutic and preventive strategies.

Introduction to Respiratory Syncytial Virus.- Quantification of Respiratory Syncytial Virus by Immunostaining Plaque Assay.- Methods for Detection, Purification, and Production of Defective Viral Genomes During Respiratory Syncytial Virus Infection.- A Rapid Yeast-Based System for Modifying Respiratory Syncytial Virus.- Respiratory Syncytial Virus Fusion Activity Syncytia Assay.- Visualization of Respiratory Syncytial Virus RNA Synthesis Sites by Ethynyl Uridine Labeling.- Minigenome System to Study Respiratory Syncytial Virus Transcription Versus Genome Replication.- Secretory Expression and Purification of Respiratory Syncytial Virus G and F Ectodomain in Human Cells.- Expression and Purification of Recombinant Respiratory Syncytial Virus Proteins.- Single Particle Electron Microscopy Analysis of Respiratory Syncytial Virus Proteins.- Single Particle Electron Microscopy Analysis of Respiratory Syncytial Virus Protein Complexes.- In Vitro Biochemical Assays to Study Respiratory Syncytial Virus Polymerase Activities.- Biophysical Characterization of the M2-1-RNA Interaction.- Biophysical Characterization of Protein-Protein Interactions in Respiratory Syncytial Virus Infections.- Bisulfite Sequencing to Map 5-Methylcytosine Sites in Human Respiratory Syncytial Virus RNA.- Well-Differentiated Primary Human Airway Epithelial Cultures for Respiratory Syncytial Virus (RSV) Infection Studies.- Utilizing Neonatal Tracheal Aspirate Derived Bronchial Basal Stem Cells to Model Respiratory Syncytial Virus Infection.- Visualizing Respiratory Syncytial Virus Replication in Live Mice.- Cotton Rat Model of Respiratory Syncytial Virus Infection.- Lamb Model of RSV Infection.- Bovine Model of Respiratory Syncytial Virus Infection.- Serial Adaptation of Viruses for Reduced Susceptibility Profiling.- Quantification of RSV Antibodies in Human Plasma by Enzyme Immunoassays.

Erscheinungsdatum	15.06.2025
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	67 Illustrations, color; 11 Illustrations, black and white
Verlagsort	New York
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizinische Fachgebiete ► Innere Medizin ► Pneumologie
	Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin
	Medizin / Pharmazie ► Medizinische Fachgebiete ► Pharmakologie / Pharmakotherapie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	RSV • Severe respiratory tract infection • vaccines • Viral detection • Viral-host interactions
ISBN-10	1-0716-4665-6 / 1071646656
ISBN-13	978-1-0716-4665-6 / 9781071646656
Zustand	Neuware