Tick-Borne Viruses
Humana (Verlag)
978-1-0716-4586-4 (ISBN)
This volume covers a variety of the latest scientific methods utilized in tick-borne virus (TBV) research. The chapters in this book explore detailed protocols on how to conduct in-depth research on TBVs; enhance understanding of the virus s biology; transmission mechanisms; and the creation of effective countermeasures. Following the established Methods in Molecular Biology series format, each chapter introduces the topic, lists necessary materials and reagents, offers step-by-step reproducible laboratory protocols, and includes troubleshooting tips to help avoid common issues.
Cutting-edge and comprehensive, Tick-Borne Viruses: Methods and Protocols is a comprehensive and innovative resource for researchers, clinicians, and public health experts interested in exploring the complexities of TBVs.
Tick Cell Culture Methods.- Virus Isolation and Culture from Field Collected Ticks: In Vitro.- Virus Isolation from Field-Collected Ticks: In Vivo.- Generation of Ticks Infected with Tick-Borne Encephalitis Virus.- Generation of Virus Infected Ticks: Synchronous Infection and Feeding on Viremic Animals.- Human Skin Ex-Vivo Culture for Tick-Borne Virus Infection Studies.- Colorado Tick Fever Virus: Quantification, Propagation, Storage, and Detection.- Isolation and Quantitation of Dabie bandavirus.- Immunofluorescence Assays to Visualize Tick-Borne Flaviviruses in Mouse Skin or Brain.- Immunohistochemical Assays to Visualize Tick-Borne Flaviviruses at the Tick Feeding Site.- RNA In-Situ Hybridization to Visualize Tick-Borne Viruses.- Quantification of Tick-Borne Flaviviruses Using Focus Forming Assays.- Quantification of Neutralizing Antibodies to Tick-Borne Viruses using Focus Neutralization Assays.- Methods for Evaluation of Antivirals and Neutralizing Antibodies Against Tick-Borne Encephalitis Virus.- Exploring Tick-Borne Encephalitis Virus-Host Cell Interactions using Electron Tomography: Methodologies and Protocols.
| Erscheinungsdatum | 01.07.2025 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology |
| Zusatzinfo | 28 Illustrations, color; 2 Illustrations, black and white |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Studium ► Querschnittsbereiche ► Infektiologie / Immunologie |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Schlagworte | immunofluorescence • Mammalian culture • micro-needle inoculation • Phlebovirus • Synchronous infection |
| ISBN-10 | 1-0716-4586-2 / 1071645862 |
| ISBN-13 | 978-1-0716-4586-4 / 9781071645864 |
| Zustand | Neuware |
| Informationen gemäß Produktsicherheitsverordnung (GPSR) | |
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